Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDLW4 – Human Protein S100-A4

Sample:	Protein S100-A4 dimer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 20 mM NaCl, 0.1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Mar 2

The C-Terminal Random Coil Region Tunes the Ca2+-Binding Affinity of S100A4 through Conformational Activation PLoS ONE 9(5):e97654 (2014)
Duelli A, Kiss B, Lundholm I, Bodor A, Petoukhov M, Svergun D, Nyitray L, Katona G, Csernoch L

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	48	nm³

SASDDF6 – Myelin basic protein

Myelin basic protein experimental SAS data

Sample:	Myelin basic protein monomer, 18 kDa Bos taurus protein
Buffer:	20 mM NaH2PO4/ Na2HPO4, 99.9% D2O, pH: 4.8
Experiment:	SAXS data collected at BM29, ESRF on 2013 Feb 21

Internal nanosecond dynamics in the intrinsically disordered myelin basic protein. J Am Chem Soc 136(19):6987-94 (2014)
Stadler AM, Stingaciu L, Radulescu A, Holderer O, Monkenbusch M, Biehl R, Richter D

R_g^Guinier	3.3	nm
D_max	11.1	nm

SASDAP4 – Chitinase 60 from Moritella marina

Chitinase 60 from Moritella marina Rg histogram

Sample:	Chitinase 60 monomer, 61 kDa Moritella marina protein
Buffer:	20 mM Tris 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 18

Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Acta Crystallogr D Biol Crystallogr 70(Pt 3):676-84 (2014)
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W

R_g^Guinier	3.2	nm
D_max	11.3	nm
Volume^Porod	75	nm³

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data

Sample:	Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	4.9	nm
D_max	15.0	nm
Volume^Porod	115	nm³

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data

Sample:	Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

R_g^Guinier	5.9	nm
D_max	17.0	nm
Volume^Porod	130	nm³

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data

Sample:	Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	56	nm³

SASDBJ4 – Wild-type LytA choline-binding domain

Lytic Amidase choline-binding domain experimental SAS data

Sample:	Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11

R_g^Guinier	3.3	nm
D_max	10.0	nm
Volume^Porod	49	nm³

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data

PsrP functional binding region Rg histogram

Sample:	Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer:	20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	2.3	nm
D_max	7.7	nm
Volume^Porod	37	nm³

SASDH23 – Antigen 43 alpha domain

Alpha domain of Ag43a experimental SAS data

Sample:	Alpha domain of Ag43a monomer, 49 kDa Escherichia coli protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2009 Nov 19

The antigen 43 structure reveals a molecular Velcro-like mechanism of autotransporter-mediated bacterial clumping. Proc Natl Acad Sci U S A 111(1):457-62 (2014)
Heras B, Totsika M, Peters KM, Paxman JJ, Gee CL, Jarrott RJ, Perugini MA, Whitten AE, Schembri MA

R_g^Guinier	3.6	nm
D_max	12.2	nm
Volume^Porod	62	nm³

SASDA35 – SeZinT-SeZnuA complex

High-affinity zinc transporter periplasmic componentZinc/cadmium-binding protein experimental SAS data

Sample:	High-affinity zinc transporter periplasmic component monomer, 33 kDa Salmonella enterica subsp. … protein Zinc/cadmium-binding protein monomer, 23 kDa Salmonella enterica subsp. … protein
Buffer:	50 mM HEPES 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 May 9

The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc. Biochim Biophys Acta 1840(1):535-44 (2014)
Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E

R_g^Guinier	2.5	nm
D_max	8.5	nm
Volume^Porod	55	nm³

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