Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDUL3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Glycosyl hydrolase family 61 experimental SAS data

Glycosyl hydrolase family 61 Kratky plot

Sample:	Glycosyl hydrolase family 61 monomer, 30 kDa Colletotrichum higginsianum (strain … protein
Buffer:	50 mM MES, 150 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 9

The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG

R_g^Guinier	4.1	nm
D_max	14.6	nm
Volume^Porod	85	nm³

SASDUM3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Sample:	Glycosyl hydrolase family 61 dimer, 61 kDa Colletotrichum higginsianum (strain … protein
Buffer:	50 mM MES, 150 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 9

R_g^Guinier	6.2	nm
D_max	24.5	nm
Volume^Porod	206	nm³

SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer

Sample:	Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein
Buffer:	50 mM MES, 150 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Feb 17

R_g^Guinier	3.9	nm
D_max	17.0	nm
Volume^Porod	68	nm³

SASDUP3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) dimer

Sample:	Endoglucanase-4 dimer, 66 kDa Colletotrichum higginsianum (strain … protein
Buffer:	50 mM MES, 150 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Feb 17

R_g^Guinier	6.4	nm
D_max	27.9	nm
Volume^Porod	199	nm³

SASDTT4 – SnoaL-like domain-containing protein apoform (SLDCP concentrated monomeric SEC peak)

SnoaL-like domain-containing protein experimental SAS data

Sample:	SnoaL-like domain-containing protein monomer, 12 kDa Sphaerobacter thermophilus (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 6

Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes. Biochim Biophys Acta Bioenerg 1865(3):149043 (2024)
Likkei K, Moldenhauer M, Tavraz NN, Egorkin NA, Slonimskiy YB, Maksimov EG, Sluchanko NN, Friedrich T

R_g^Guinier	1.5	nm
D_max	5.0	nm
Volume^Porod	18	nm³

SASDKT6 – SARS-CoV-2 non-structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data

Sample:	Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A

R_g^Guinier	2.7	nm
D_max	9.6	nm
Volume^Porod	80	nm³

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data

Sample:	Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	2.9	nm
D_max	12.2	nm
Volume^Porod	73	nm³

SASDKV6 – SARS-CoV-2 non-structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data

Sample:	Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	2.0	nm
D_max	8.0	nm
Volume^Porod	28	nm³

SASDKW6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data

Sample:	Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	4.7	nm
D_max	11.4	nm
Volume^Porod	113	nm³

SASDTV5 – Human full-length Annexin A11 (apo form)

Sample:	Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM KCl, 5 mM EDTA, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jul 21

The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
Dudas E, Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A

R_g^Guinier	3.8	nm
D_max	15.9	nm
Volume^Porod	84	nm³

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