Browse by MACROMOLECULE type: protein

SASDLX2 – Immunoglobulin G4 (IgG4) Deglycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4. PLoS One 19(4):e0300964 (2024)
Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ
RgGuinier 4.9 nm
Dmax 16.9 nm
VolumePorod 258 nm3

SASDLY2 – Immunoglobulin G4 (IgG4) Glycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4. PLoS One 19(4):e0300964 (2024)
Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ
RgGuinier 4.9 nm
Dmax 16.3 nm
VolumePorod 256 nm3

SASDNK8 – The C-terminal region of histone-lysine N-methyltransferase NSD3: SET-PHD4 construct

Histone-lysine N-methyltransferase NSD3 experimental SAS data
OTHER model
Sample: Histone-lysine N-methyltransferase NSD3 monomer, 40 kDa Homo sapiens protein
Buffer: 0.5 M NaCl, 20 mM Tris-HCl, 5 mM DTT, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Nov 25
Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering. Front Mol Biosci 11:1191246 (2024)
Belviso BD, Shen Y, Carrozzini B, Morishita M, di Luccio E, Caliandro R
RgGuinier 3.4 nm
Dmax 13.2 nm
VolumePorod 75 nm3

SASDNL8 – The C-terminal region of histone-lysine N-methyltransferase NSD3: PWWP2-SET construct

Histone-lysine N-methyltransferase NSD3 experimental SAS data
OTHER model
Sample: Histone-lysine N-methyltransferase NSD3 monomer, 43 kDa Homo sapiens protein
Buffer: 0.5 M NaCl, 20 mM Tris-HCl, 5 mM DTT, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Nov 25
Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering. Front Mol Biosci 11:1191246 (2024)
Belviso BD, Shen Y, Carrozzini B, Morishita M, di Luccio E, Caliandro R
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 64 nm3

SASDNG9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 (apo conditions)

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 1.8 nm
Dmax 6.1 nm
VolumePorod 33 nm3

SASDNH9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 with GDP

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 38 nm3

SASDNJ9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 with GTP

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 2.0 nm
Dmax 7.7 nm
VolumePorod 38 nm3

SASDTG2 – LCP domain of LytR from Streptococcus dysgalactiae in the presence of geranylgeranyl diphosphate (150 mM NaCl)

Biofilm regulatory protein experimental SAS data
Biofilm regulatory protein Kratky plot
Sample: Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 1
Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
Paquete-Ferreira J, Freire F, Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T
RgGuinier 2.5 nm
Dmax 8.0 nm
VolumePorod 83 nm3

SASDTH2 – LCP domain of LytR from Streptococcus dysgalactiae in the absence of ligand (150 mM NaCl)

Biofilm regulatory protein experimental SAS data
Biofilm regulatory protein Kratky plot
Sample: Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 1
Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
Paquete-Ferreira J, Freire F, Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 76 nm3

SASDTU4 – 24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
CORAL model
Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) 24-mer, 755 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 30
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2010 nm3