Browse by MACROMOLECULE type: protein

SASDN69 – Complex glycan contactin-2 ectodomain, 6 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.0 nm
Dmax 70.0 nm
VolumePorod 1900 nm3

SASDN79 – Complex glycan contactin-2 ectodomain, 12 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.3 nm
Dmax 66.0 nm
VolumePorod 1820 nm3

SASDN89 – Complex glycan contactin-2 ectodomain, 23 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 12.5 nm
Dmax 65.0 nm
VolumePorod 2044 nm3

SASDN99 – Complex glycan contactin-2 ectodomain, 50 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 14.2 nm
Dmax 65.0 nm
VolumePorod 2190 nm3

SASDPQ5 – His-tagged L-methionine gamma-lyase from Clostridium tetani

L-methionine gamma-lyase experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase tetramer, 181 kDa Clostridium tetani protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 4.0 nm
Dmax 14.9 nm
VolumePorod 232 nm3

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes

L-methionine gamma-lyase (K272S) experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase (K272S) tetramer, 174 kDa Clostridium sporogenes protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 211 nm3

SASDPS5 – L-methionine gamma-lyase from Clostridium sporogenes fused with S3 domain of the Vaccinia virus growth factor

L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain tetramer, 183 kDa Clostridium sporogenes protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 5.2 nm
Dmax 17.3 nm
VolumePorod 303 nm3

SASDSH5 – UDP-glycosyltransferase 202A2 from Tetranychus urticae

UDP-glycosyltransferase 202A2 experimental SAS data
MULTIFOXS model
Sample: UDP-glycosyltransferase 202A2 monomer, 53 kDa Tetranychus urticae protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 17
Structural and functional studies reveal the molecular basis of substrate promiscuity of a glycosyltransferase originating from a major agricultural pest Journal of Biological Chemistry :105421 (2023)
Arriaza R, Abiskaroon B, Patel M, Daneshian L, Kluza A, Snoeck S, Watkins M, Hopkins J, Van Leeuwen T, Grbic M, Grbic V, Borowski T, Chruszcz M
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDSX8 – Solution scattering studies on 20 kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis (ISPC-12)

20 kDa accessory protein experimental SAS data
GASBOR model
Sample: 20 kDa accessory protein dimer, 42 kDa Bacillus thuringiensis serovar … protein
Buffer: 10 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2023 Aug 15
20-kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis ISPC-12: Purification, characterization, solution scattering and structural analysis International Journal of Biological Macromolecules :127985 (2023)
Kinkar O, Singh R, Prashar A, Kumar A, Hire R, Makde R
RgGuinier 3.0 nm
Dmax 8.6 nm
VolumePorod 67 nm3

SASDTX4 – G-actin - Skeletal muscle actin from Oryctolagus cuniculus: globular actin monomers

Actin, alpha skeletal muscle experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1
Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications :149340 (2023)
Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I
RgGuinier 2.9 nm
Dmax 8.5 nm
VolumePorod 58 nm3