Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDTY4 – F-actin - Skeletal muscle actin from Oryctolagus cuniculus: fibrillar actin

Actin, alpha skeletal muscle experimental SAS data

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 1.0 mM ATP, 50 mM KCl, 2 mM MgCl2, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1

Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications :149340 (2023)
Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I

R_g^Guinier	15.7	nm
D_max	60.0	nm
Volume^Porod	4710	nm³

SASDTZ4 – I-actin - Skeletal muscle actin from Oryctolagus cuniculus: oligomer of time-inactivated actin (peak SEC fraction)

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris/Tris-HCl, 2.0 mM EDTA, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Nov 16

R_g^Guinier	4.9	nm
D_max	22.0	nm
Volume^Porod	549	nm³

SASDT25 – G- / I-actin mixture - Skeletal muscle actin from Oryctolagus cuniculus: mixture of globular actin monomers and inactivated actin monomers and dimers

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 5

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDSH9 – SAXS data of Clostridium perfringens enterotoxin

Heat-labile enterotoxin B chain experimental SAS data

Sample:	Heat-labile enterotoxin B chain monomer, 36 kDa Clostridium perfringens protein
Buffer:	10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18

Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A

R_g^Guinier	2.7	nm
D_max	10.2	nm
Volume^Porod	46	nm³

SASDSJ9 – SAXS data of trypsinized Clostridium perfringens enterotoxin

Sample:	Heat-labile enterotoxin B chain monomer, 33 kDa Clostridium perfringens protein
Buffer:	10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18

Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	40	nm³

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

Heat shock factor 2-binding protein experimental SAS data

Heat shock factor 2-binding protein Kratky plot

Sample:	Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Oct 2

BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
Ghouil R, Miron S, Sato K, Ristic D, van Rossum-Fikkert SE, Legrand P, Ouldali M, Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S

R_g^Guinier	8.5	nm
D_max	25.0	nm
Volume^Porod	364	nm³

SASDTU2 – NAD-dependent protein deacetylase sirtuin-7 at pH 8.0

Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 experimental SAS data

Sample:	Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 monomer, 45 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Dec 16

Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide-Activated Sirtuin 7. Angew Chem Int Ed Engl :e202314597 (2023)
Bolding JE, Nielsen AL, Jensen I, Hansen TN, Ryberg LA, Jameson ST, Harris P, Peters GHJ, Denu JM, Rogers JM, Olsen CA

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	89	nm³

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

R_g^Guinier

3.1

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

5.8

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