Browse by MACROMOLECULE type: protein

SASDRS4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) dimer

Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 dimer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 141 nm3

SASDRT4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer

Mycobacterium tuberculosis phosphoserine phosphatase SerB2 experimental SAS data
DADIMODO model
Sample: Mycobacterium tuberculosis phosphoserine phosphatase SerB2 tetramer, 173 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 12.5 nm
VolumePorod 307 nm3

SASDRU4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) trimer formed in the presence of L-serine

Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 200 nm3

SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

phosphoserine phosphatase experimental SAS data
phosphoserine phosphatase Kratky plot
Sample: Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 138 nm3

SASDRW4 – Mycobacterium avium phosphoserine phosphatase (MaSerB) dimer

Phosphoserine phosphatase (G31R, G152E) experimental SAS data
DADIMODO model
Sample: Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 138 nm3

SASDS46 – Human phosphorylated calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1) bound to 14-3-3 protein gamma

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L)14-3-3 protein gamma experimental SAS data
CORAL model
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 152 nm3

SASDSJ7 – Human C-terminally truncated 14-3-3 gamma

14-3-3 protein gamma experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 76 nm3

SASDSQ7 – Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
MULTIFOXS model
Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 91 nm3

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma

14-3-3 protein gammaCalcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
ALLOSMOD model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 157 nm3

SASDSR8 – Calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 114 nm3