Browse by ORGANISM: Homo sapiens (Human)

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.8 nm
Dmax 20.2 nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.4 nm
Dmax 18.3 nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm

SASDEG5 – apo human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 300 nm3

SASDEH5 – AMP/fumarate-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 256 nm3

SASDEJ5 – AICAR/fumarate-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 250 nm3