Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDCP6 – Small GTPase Rab5 conjugated with ubiquitin at K165

Monoubiquitinated Rab5 at K165 experimental SAS data

Small GTPase Rab5 conjugated with ubiquitin at K165 Rg histogram

Sample:	Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21

Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S

R_g^Guinier	2.7	nm
D_max	9.4	nm

SASDCC3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA

Nucleolysin TIA-1 isoform p40poly U 15mer experimental SAS data

Nucleolysin TIA-1 isoform p40 poly U 15mer Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein Poly U 15mer monomer, 5 kDa RNA
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M

R_g^Guinier	2.4	nm
D_max	8.7	nm
Volume^Porod	39	nm³

SASDCD3 – Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)

Nucleolysin TIA-1 isoform p40 experimental SAS data

Nucleolysin TIA-1 isoform p40 Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1

R_g^Guinier	2.7	nm
D_max	10.3	nm
Volume^Porod	38	nm³

SASDCE3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)

poly U 15merNucleolysin TIA-1 isoform p40 experimental SAS data

poly U 15mer Nucleolysin TIA-1 isoform p40 Kratky plot

Sample:	Poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1

R_g^Guinier	2.4	nm
D_max	8.6	nm
Volume^Porod	40	nm³

SASDCF3 – Nucleolysin TIA-1 isoform p40

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28

R_g^Guinier	2.7	nm
D_max	11.2	nm
Volume^Porod	38	nm³

SASDCG3 – Nucleolysin TIA-1 isoform p40 (LPATG containing construct for sortase mediated protein ligation)

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4

R_g^Guinier	2.7	nm
D_max	10.5	nm
Volume^Porod	37	nm³

SASDCH3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPATG containing construct for sortase mediated protein ligation)

Sample:	Poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4

R_g^Guinier	2.4	nm
D_max	8.6	nm
Volume^Porod	40	nm³

SASDER2 – Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1 experimental SAS data

Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant Rg histogram

Sample:	Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	30	nm³

SASDES2 – Labeled Importin Beta Binding Domain (IBB-Alexa488/Alexa594) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 9

R_g^Guinier	2.9	nm
D_max	13.7	nm
Volume^Porod	29	nm³

SASDET2 – Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant

Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant Rg histogram

Sample:	Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	3.1	nm
D_max	15.3	nm
Volume^Porod	34	nm³

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