Browse by ORGANISM: Homo sapiens (Human)

SASDC26 – DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein (DH-PH) experimental SAS data
DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 Rg histogram
Sample: BCR-ABL p210 fusion protein (DH-PH) monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13
Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 8(1):2101 (2017)
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 69 nm3

SASDC36 – DH - Dbl-homology domain of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: BCR-ABL p210 fusion protein monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13
Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 8(1):2101 (2017)
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 38 nm3

SASDC46 – PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein (PH domain) experimental SAS data
SREFLEX model
Sample: BCR-ABL p210 fusion protein (PH domain) monomer, 22 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 10
Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 8(1):2101 (2017)
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 38 nm3

SASDB79 – Basic domain of human telomeric repeat-binding factor 2 (TRF2) in complex with telomeric DNA duplex

Basic domain of telomeric repeat-binding factor 2telomere DNA duplex experimental SAS data
MONSA model
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
Telomere DNA duplex monomer, 11 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 6.0 nm
VolumePorod 20 nm3

SASDC62 – Basic domain of telomeric repeat-binding factor 2 (TRF2)

Basic domain of telomeric repeat-binding factor 2 experimental SAS data
Basic domain of telomeric repeat-binding factor 2 Kratky plot
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
Buffer: 50 mM NaPi, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2015 Mar 5
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 4 nm3

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3

SASDC76 – Extracellular domain of human B-lymphocyte cell receptor CD22

CD22 extracellular domain experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.0 nm
Dmax 30.6 nm

SASDC86 – Extracellular domain of human B-lymphocyte cell receptor CD22 in complex with alpha 2,6 sialyllactose

CD22 extracellular domainalpha(2,6)-Sialyllactose experimental SAS data
DAMMIF model
Sample: CD22 extracellular domain monomer, 90 kDa Homo sapiens protein
Alpha(2,6)-Sialyllactose, 1 kDa
Buffer: 20 mM Tris 150 mM NaCl, pH: 9
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 21
Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8(1):764 (2017)
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP
RgGuinier 8.1 nm
Dmax 29.8 nm

SASDCM6 – Small GTPase Rab5 conjugated with ubiquitin at K116

Monoubiquitinated Rab5 at K165 experimental SAS data
Small GTPase Rab5 conjugated with ubiquitin at K116 Rg histogram
Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
RgGuinier 2.6 nm
Dmax 8.7 nm

SASDCN6 – Small GTPase Rab5 conjugated with ubiquitin at K140

Monoubiquitinated Rab5 at K165 experimental SAS data
Small GTPase Rab5 conjugated with ubiquitin at K140 Rg histogram
Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
RgGuinier 2.3 nm
Dmax 8.4 nm