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SASDHS4 – Protein ninH from Bacteriophage lambda (N-terminal histidine tagged) T15A mutated

Protein ninH experimental SAS data
MODELLER model
Sample: Protein ninH dimer, 20 kDa Escherichia phage lambda protein
Buffer: 150 mM NaCl, 50 mM phosphate buffer (pH 7.4), 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 1
A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochem J (2020)
Chakraborti S, Balakrishnan D, Trotter AJ, Gittens WH, Yang AWH, Jolma A, Paterson JR, Świątek S, Plewka J, Curtis FA, Bowers LY, Pålsson LO, Hughes TR, Taube M, Kozak M, Heddle JG, Sharples GJ
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 33 nm3

SASDGV2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 87 nm3

SASDGW2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 210 nm3

SASDGX2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H st-sR26 RNA, 1H substrate D' and 2H L7Ae (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Nov 23
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 42 nm3

SASDGY2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H L7Ae, 1H substrate D' and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jul 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 52 nm3

SASDGZ2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H substrate D', 2H Fibrillarin and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 23
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.8 nm
Dmax 16.6 nm
VolumePorod 82 nm3

SASDG23 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D', 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.8 nm
VolumePorod 214 nm3

SASDG33 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D, 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 231 nm3

SASDG43 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.6 nm
VolumePorod 65 nm3

SASDG53 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 222 nm3