Browse by ORGANISM: other species

SASDHF9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) M-domain

Plasmodium falciparum Heat shock protein 90 middle domain experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 middle domain monomer, 33 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
Silva N, Torricillas M, Minari K, Barbosa L, Seraphim T, Borges J
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 47 nm3

SASDJR6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)

Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) experimental SAS data
DAMFILT model
Sample: Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 23 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES, 100 mM NaCl, 0.4 mM EGTA, 2.4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 25
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 2.6 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDG75 – Pomacea diffusa perivitellin-1

Pomacea diffusa perivitellin-1 experimental SAS data
DAMMIF model
Sample: Pomacea diffusa perivitellin-1, 23 kDa Pomacea diffusa protein
Buffer: 50 mM Phosphate buffer, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 Mar 27
A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs. J Exp Biol 223(Pt 19) (2020)
Brola TR, Dreon MS, Qiu JW, Heras H
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 529 nm3

SASDHG4 – NopAA, a type three effector from Sinorhizobium fredii USDA257 whith xyloglucanase activity

Type III effector NopAA experimental SAS data
ALLOSMOD model
Sample: Type III effector NopAA monomer, 31 kDa Sinorhizobium fredii USDA257 protein
Buffer: PBS, 150 mM NaCl, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 17
Structural and enzymatic characterisation of the Type III effector NopAA (=GunA) from Sinorhizobium fredii USDA257 reveals a Xyloglucan hydrolase activity. Sci Rep 10(1):9932 (2020)
Dorival J, Philys S, Giuntini E, Brailly R, de Ruyck J, Czjzek M, Biondi E, Bompard C
RgGuinier 2.4 nm
Dmax 9.9 nm
VolumePorod 38 nm3

SASDH55 – Modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot
Sample: Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 94 nm3

SASDHW2 – The Legionella pneumophila type II secretion system substrate NttE

Uncharacterized protein experimental SAS data
GASBOR model
Sample: Uncharacterized protein dimer, 66 kDa Legionella pneumophila protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18
Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System Frontiers in Molecular Biosciences 7 (2020)
Portlock T, Tyson J, Dantu S, Rehman S, White R, McIntire I, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto N, Garnett J
RgGuinier 2.9 nm
Dmax 9.4 nm
VolumePorod 114 nm3

SASDFW7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆328-352 (MCoA∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) experimental SAS data
DAMFILT model
Sample: Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 13
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
Borges P, Brissos V, Hernandez G, Masgrau L, Lucas M, Monza E, Frazão C, Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 6.9 nm
VolumePorod 77 nm3

SASDFX7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆337-346 (MCoA∆337-346)

Aquifex aeolicus McoA metaloxidase ∆337-346 experimental SAS data
DAMMIF model
Sample: Aquifex aeolicus McoA metaloxidase ∆337-346 monomer, 54 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 4
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
Borges P, Brissos V, Hernandez G, Masgrau L, Lucas M, Monza E, Frazão C, Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 78 nm3

SASDFY7 – Aquifex aeolicus McoA metaloxidase

Aquifex aeolicus McoA metaloxidase experimental SAS data
DAMFILT model
Sample: Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 15
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
Borges P, Brissos V, Hernandez G, Masgrau L, Lucas M, Monza E, Frazão C, Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 79 nm3

SASDH92 – Plasmodium falciparum lipocalin (PF3D7_0925900): Dimer-tetramer equilibrium through a concentration series (combined batch and SEC-SAXS measurements)

Plasmodium falciparum Lipocalin experimental SAS data
SASREF MX model
Sample: Plasmodium falciparum Lipocalin tetramer, 89 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris pH7.5, 150 mM NaCl, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structure-Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum Cell Reports 31(12):107817 (2020)
Burda P, Crosskey T, Lauk K, Zurborg A, Söhnchen C, Liffner B, Wilcke L, Pietsch E, Strauss J, Jeffries C, Svergun D, Wilson D, Wilmanns M, Gilberger T
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 126 nm3