Browse by ORGANISM: other species

SASDD42 – Synechocystis fluorescence recovery protein SynFRP.8-109

Fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Fluorescence recovery protein dimer, 23 kDa Synechocystis sp. PCC … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP. Biochim Biophys Acta 1859(5):382-393 (2018)
Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 36 nm3

SASDD52 – Arthrospira fluorescence recovery protein AmaxFRP.1-106

Uncharacterized fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Uncharacterized fluorescence recovery protein dimer, 24 kDa Arthrospira maxima CS-328 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP. Biochim Biophys Acta 1859(5):382-393 (2018)
Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDD65 – Ubiquitinating/deubiquitinating enzyme SdeA 207 914

Ubiquitinating/deubiquitinating enzyme SdeA experimental SAS data
OTHER model
Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 72 kDa Legionella pneumophila subsp. … protein
Buffer: 10 mM HEPES 150 mM NaCl 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination. Nature 557(7707):734-738 (2018)
Kalayil S, Bhogaraju S, Bonn F, Shin D, Liu Y, Gan N, Basquin J, Grumati P, Luo ZQ, Dikic I
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 128 nm3

SASDC48 – ScsC-ScsBalpha complex

DsbA-like proteinPutative metal resistance protein experimental SAS data
SASREF model
Sample: DsbA-like protein trimer, 74 kDa Proteus mirabilis protein
Putative metal resistance protein monomer, 30 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 2
Disulfide isomerase activity of the dynamic, trimeric Proteus mirabilis ScsC protein is primed by the tandem immunoglobulin-fold domain of ScsB. J Biol Chem 293(16):5793-5805 (2018)
Furlong EJ, Choudhury HG, Kurth F, Duff AP, Whitten AE, Martin JL
RgGuinier 3.9 nm
Dmax 11.5 nm
VolumePorod 145 nm3

SASDCH9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) with GDP

Sensory box/response regulator experimental SAS data
CORAL model
Sample: Sensory box/response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis. Biochem J 475(7):1295-1308 (2018)
Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q
RgGuinier 5.0 nm
Dmax 20.0 nm
VolumePorod 299 nm3

SASDCJ9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP

Sensory box/response regulator experimental SAS data
CORAL model
Sample: Sensory box/response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis. Biochem J 475(7):1295-1308 (2018)
Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 271 nm3

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii

Calvin cycle protein CP12, chloroplastic experimental SAS data
Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Buffer: 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 3
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 22 nm3

SASDCZ9 – Glyceraldehyde-3-phosphate dehydrogenase from C. reinhardtii

Glyceraldehyde-3-phosphate dehydrogenase experimental SAS data
MODELLER model
Sample: Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 7
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 206 nm3

SASDD22 – Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

Calvin cycle protein CP12, chloroplasticGlyceraldehyde-3-phosphate dehydrogenase experimental SAS data
Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2010 Dec 8
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.8 nm
Dmax 19.0 nm
VolumePorod 251 nm3

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3