Browse by ORGANISM: other species

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDCT8 – Uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
DAMMIF model
Sample: Hypothetical protein CTHT_0072540 tetramer, 62 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 30
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 4.1 nm
Dmax 16.2 nm
VolumePorod 145 nm3

SASDCU8 – Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: Hypothetical protein CTHT_0072540 monomer, 35 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 19
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 2.3 nm
Dmax 5.8 nm
VolumePorod 68 nm3

SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum

Full-length hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: Full-length hypothetical protein CTHT_0072540 tetramer, 207 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Oct 15
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 280 nm3

SASDC67 – SaPIbov1 pathogenicity island repressor

SaPIbov1 pathogenicity island repressor experimental SAS data
OTHER model
Sample: SaPIbov1 pathogenicity island repressor dimer, 64 kDa Staphylococcus aureus protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 9
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
Nyíri K, Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 100 nm3

SASDC55 – Plasmodium falciparum p23A

CS domain protein, putative experimental SAS data
DAMFILT model
Sample: CS domain protein, putative monomer, 19 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
Silva NSM, Seraphim TV, Minari K, Barbosa LRS, Borges JC
RgGuinier 2.5 nm
Dmax 8.5 nm

SASDC65 – Plasmodium falciparum p23B

Co-chaperone p23 experimental SAS data
DAMFILT model
Sample: Co-chaperone p23 monomer, 31 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
Silva NSM, Seraphim TV, Minari K, Barbosa LRS, Borges JC
RgGuinier 3.7 nm
Dmax 13.0 nm

SASDCB3 – Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

methylated C-terminal ZBTB38 binding sequence experimental SAS data
DAMMIN model
Sample: Methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Oct 27
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDLM4 – Prebiotic-producing GH10 xylanase (RmXyn10A, full length)

Prebiotic-producing GH10 xylanase (RmXyn10A, full length) experimental SAS data
BUNCH model
Sample: Prebiotic-producing GH10 xylanase (RmXyn10A, full length) monomer, 108 kDa Rhodothermus marinus protein
Buffer: 50 mM TRIS, 0.5 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Mar 12
Structural insights of RmXyn10A - A prebiotic-producing GH10 xylanase with a non-conserved aglycone binding region. Biochim Biophys Acta Proteins Proteom 1866(2):292-306 (2018)
Aronsson A, Güler F, Petoukhov MV, Crennell SJ, Svergun DI, Linares-Pastén JA, Nordberg Karlsson E
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 170 nm3

SASDDQ2 – EspG3 chaperone from Mycobacterium marinum

EspG3 chaperone from Mycobacterium marinum M experimental SAS data
EspG3 chaperone from Mycobacterium marinum M Kratky plot
Sample: EspG3 chaperone from Mycobacterium marinum M monomer, 32 kDa Mycobacterium marinum M protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.3 nm
Dmax 8.0 nm