Small Angle Scattering Biological Data Bank SASBDB

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SASDWP4 – Shewanella benthica hemoglobin 100 MPa

Group 1 truncated hemoglobin (C51S, C71S) experimental SAS data

Group 1 truncated hemoglobin (C51S, C71S) Kratky plot

Sample:	Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N Journal of Biological Chemistry :108223 (2025)
Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J

R_g^Guinier	2.2	nm
D_max	6.4	nm
Volume^Porod	50	nm³

SASDWQ4 – Shewanella benthica hemoglobin 150 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	50	nm³

SASDWR4 – Shewanella benthica hemoglobin 200 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	41	nm³

SASDWS4 – Shewanella benthica hemoglobin 250 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.0	nm
D_max	8.0	nm
Volume^Porod	28	nm³

SASDWT4 – Shewanella benthica hemoglobin 350 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.2	nm
D_max	9.0	nm
Volume^Porod	30	nm³

SASDWU4 – Shewanella benthica hemoglobin K111I 0.101 MPa

Group 1 truncated hemoglobin (C51S, C71S, K111I) experimental SAS data

Group 1 truncated hemoglobin (C51S, C71S, K111I) Kratky plot

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.9	nm
D_max	7.0	nm
Volume^Porod	38	nm³

SASDWV4 – Shewanella benthica hemoglobin K111I 0.101 MPa (final measurement after end of pressure series)

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.7	nm
D_max	6.3	nm
Volume^Porod	28	nm³

SASDWW4 – Shewanella benthica hemoglobin K111I 50 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.6	nm
D_max	6.0	nm
Volume^Porod	24	nm³

SASDWX4 – Shewanella benthica hemoglobin K111I 100 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.6	nm
D_max	5.7	nm
Volume^Porod	25	nm³

SASDWY4 – Shewanella benthica hemoglobin K111I 150 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	24	nm³

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