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SASDRK5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 10 nm3

SASDRL5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.2 nm
VolumePorod 29 nm3

SASDRM5 – A ssr1698 Dri1 hemoprotein, wild-type (Co2+) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDS76 – Antitoxin HigA-2

Antitoxin HigA-2 experimental SAS data
Antitoxin HigA-2 Kratky plot
Sample: Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Apr 6
Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
Hadži S, Živič Z, Kovačič M, Zavrtanik U, Haeserts S, Charlier D, Plavec J, Volkov AN, Lah J, Loris R
RgGuinier 2.3 nm
Dmax 9.6 nm
VolumePorod 43 nm3

SASDS86 – Antitoxin HigA-2 in complex with a DNA operator sequence

Antitoxin HigA-2DNA operator experimental SAS data
Antitoxin HigA-2 DNA operator Kratky plot
Sample: Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
DNA operator dimer, 20 kDa DNA
Buffer: 20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Apr 6
Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
Hadži S, Živič Z, Kovačič M, Zavrtanik U, Haeserts S, Charlier D, Plavec J, Volkov AN, Lah J, Loris R
RgGuinier 2.7 nm
Dmax 9.6 nm
VolumePorod 57 nm3

SASDTT7 – Lactoferrin binding protein B

Transferrin-binding protein B experimental SAS data
Transferrin-binding protein B Kratky plot
Sample: Transferrin-binding protein B monomer, 97 kDa Moraxella bovis protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23
A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase separated droplets PNAS Nexus (2024)
Ostan N, Cole G, Wang F, Reichheld S, Moore G, Pan C, Yu R, Lai C, Sharpe S, Lee H, Schryvers A, Moraes T
RgGuinier 3.7 nm
Dmax 12.4 nm
VolumePorod 130 nm3

SASDTU7 – Lactoferrin binding protein B (C-terminal alpha helical domain)

Transferrin-binding protein B experimental SAS data
Transferrin-binding protein B Kratky plot
Sample: Transferrin-binding protein B monomer, 32 kDa Moraxella bovis protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23
A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase separated droplets PNAS Nexus (2024)
Ostan N, Cole G, Wang F, Reichheld S, Moore G, Pan C, Yu R, Lai C, Sharpe S, Lee H, Schryvers A, Moraes T
RgGuinier 3.2 nm
Dmax 11.9 nm
VolumePorod 49 nm3

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – Light-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3

SASDUG3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 50 nm3