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SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data
BILBOMD model
Sample: Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 170 nm3

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data
BILBOMD model
Sample: Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 149 nm3

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

RTX toxin transporter experimental SAS data
GASBOR model
Sample: RTX toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer: 100 mM HEPES, 10 % glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 48 nm3

SASDQS9 – A ssr1698 Dri1 hemoprotein, H21A variant + heme

Ssr1698 protein (H21A) experimental SAS data
Sample: Ssr1698 protein (H21A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Dec 7
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 28 nm3

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme

Ssr1698 protein (H79A:R90A) experimental SAS data
Sample: Ssr1698 protein (H79A:R90A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 28 nm3

SASDRE5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant

Ssr1698 protein (H79A:R90A) experimental SAS data
Sample: Ssr1698 protein (H79A:R90A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 12 nm3

SASDRF5 – A ssr1698 Dri1 hemoprotein, H21A variant

Ssr1698 protein (H21A) experimental SAS data
Sample: Ssr1698 protein (H21A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 10
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 12 nm3

SASDRG5 – A ssr1698 Dri1 hemoprotein, wild-type (apo) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDRH5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Dec 12
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.3 nm
VolumePorod 12 nm3

SASDRJ5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 26 nm3