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SASDVW4 – ST9a RNA, WT (54 nucleotides in 1 mM MgCl2; monomer)

ST9 WT (54 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (54 nt RNA) monomer, 17 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Mar 23
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 1.7 nm
Dmax 5.8 nm
VolumePorod 20 nm3

SASDVX4 – ST9a RNA, WT (59 nucleotides in 10 mM MgCl2; monomer)

ST9 WT (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 1.9 nm
Dmax 6.7 nm
VolumePorod 24 nm3

SASDVY4 – ST9a RNA, WT (59 nucleotides in 0 mM MgCl2; monomer)

ST9 WT (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 26 nm3

SASDVZ4 – ST9a RNA, PKmutant (59 nucleotides in 10 mM MgCl2; dimer)

ST9 PKmutant (59 nt RNA) experimental SAS data
ST9 PKmutant (59 nt RNA) Kratky plot
Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 3.5 nm
Dmax 12.8 nm
VolumePorod 62 nm3

SASDV25 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; monomer)

ST9 PKmutant (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 PKmutant (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 29 nm3

SASDV35 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; dimer)

ST9 PKmutant (59 nt RNA) experimental SAS data
ST9 PKmutant (59 nt RNA) Kratky plot
Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
A conserved viral RNA fold enables nuclease resistance across kingdoms of life. Nucleic Acids Res 53(16) (2025)
Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
RgGuinier 3.8 nm
Dmax 12.5 nm
VolumePorod 73 nm3

SASDXX5 – SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.8 nm
Dmax 17.1 nm

SASDXY5 – SARS-CoV-2 Nucleocapsid protein 1 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 18.8 nm

SASDXZ5 – SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.7 nm
Dmax 18.8 nm

SASDX26 – SARS-CoV-2 Nucleocapsid protein 3 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 6.3 nm
Dmax 23.7 nm