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SASDX36 – SARS-CoV-2 Nucleocapsid protein 4 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 6.6 nm
Dmax 22.5 nm

SASDX46 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 15.0 nm

SASDX56 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.4 nm
Dmax 166.0 nm

SASDX66 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 19.3 nm

SASDX76 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 2 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.5 nm
Dmax 17.8 nm

SASDX86 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 3 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.9 nm
Dmax 19.8 nm

SASDX96 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 4 mg/mL

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.9 nm
Dmax 23.5 nm

SASDWB7 – Dynamin central region family protein from Leishmania donovani DLP1 G354D / R357S

Dynamin central region family protein experimental SAS data
BILBOMD model
Sample: Dynamin central region family protein dimer, 165 kDa Leishmania donovani protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 4
Biophysical analysis of an oligomerization-attenuated variant of the Leishmania donovani dynamin-1-like protein. Mol Biochem Parasitol 263:111691 (2025)
Wuyts E, Sundaramoorthy R, Tulloch L, Monsieurs P, Eadsforth TC, Pintelon I, Timmermans JP, Dujardin JC, Domagalska MA, Caljon G, De Rycker M, Postis VLG, Wyllie S, Sterckx YG
RgGuinier 6.6 nm
Dmax 25.0 nm
VolumePorod 340 nm3

SASDRK9 – single self-amplifying RNA

self-amplifying RNA experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10
Compact polyethylenimine-complexed mRNA vaccines Nature Nanotechnology (2025)
Moreno Herrero J, Stahl T, Erbar S, Maxeiner K, Schlegel A, Bacic T, Schumacher J, Cavalcanti L, Schroer M, Svergun D, Sahin U, Haas H
RgGuinier 90.5 nm
Dmax 200.0 nm

SASDRL9 – single self-amplifying RNA + 50 mM NaCl

self-amplifying RNA experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 10
Compact polyethylenimine-complexed mRNA vaccines Nature Nanotechnology (2025)
Moreno Herrero J, Stahl T, Erbar S, Maxeiner K, Schlegel A, Bacic T, Schumacher J, Cavalcanti L, Schroer M, Svergun D, Sahin U, Haas H
RgGuinier 30.7 nm
Dmax 107.4 nm
VolumePorod 112000 nm3