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SASDKJ5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKK5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKL5 – RNA Short Tetraloop Hairpin Triplex

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 6.3 nm

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA experimental SAS data
30nt Single-stranded RNA Kratky plot
Sample: 30nt Single-stranded RNA monomer, 10 kDa RNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.2 nm
Dmax 8.2 nm

SASDN57 – beta-hexosyl transferase (23-594), 1 mg/mL

Beta-galactosidase-like enzyme experimental SAS data
Beta-galactosidase-like enzyme Kratky plot
Sample: Beta-galactosidase-like enzyme dimer, 129 kDa Hamamotoa singularis protein
Buffer: 5 mM sodium phosphate, pH: 5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Oak Ridge National Laboratory on 2018 Jun 25
Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis Frontiers in Bioengineering and Biotechnology 11 (2023)
Dagher S, Vaishnav A, Stanley C, Meilleur F, Edwards B, Bruno-Bárcena J
RgGuinier 3.9 nm
Dmax 12.4 nm
VolumePorod 238 nm3

SASDN67 – beta-hexosyl transferase (23-594), 4 mg/mL

Beta-galactosidase-like enzyme experimental SAS data
Beta-galactosidase-like enzyme Kratky plot
Sample: Beta-galactosidase-like enzyme dimer, 129 kDa Hamamotoa singularis protein
Buffer: 5 mM sodium phosphate, pH: 5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Oak Ridge National Laboratory on 2018 Jun 25
Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis Frontiers in Bioengineering and Biotechnology 11 (2023)
Dagher S, Vaishnav A, Stanley C, Meilleur F, Edwards B, Bruno-Bárcena J
RgGuinier 3.7 nm
Dmax 12.5 nm
VolumePorod 236 nm3

SASDRF8 – Plasmodium falciparum circumsporozoite protein N-terminal domain at pH 6.6

Circumsporozoite protein experimental SAS data
Circumsporozoite protein Kratky plot
Sample: Circumsporozoite protein monomer, 11 kDa Plasmodium falciparum (isolate … protein
Buffer: 50 mM MES, 500 mM NaCl, 1 mM TCEP, pH: 6.6
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain Protein Science (2023)
Geens R, Stanisich J, Beyens O, D'Hondt S, Thiberge J, Ryckebosch A, Groot A, Magez S, Vertommen D, Amino R, Winter H, Volkov A, Tompa P, Sterckx Y
RgGuinier 2.3 nm
Dmax 9.1 nm
VolumePorod 24 nm3

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.2 nm
Dmax 17.7 nm
VolumePorod 296 nm3

SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)

Condensin complex subunit 1Condensin complex subunit 2, 336-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 336-418 monomer, 9 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.0 nm
Dmax 16.9 nm
VolumePorod 279 nm3

SASDJ32 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 225-418 (Brn1L)

Condensin complex subunit 1Condensin complex subunit 2, 225-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 4.9 nm
Dmax 15.9 nm
VolumePorod 309 nm3