Small Angle Scattering Biological Data Bank SASBDB

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SASDKU9 – Thymidylate kinase (dTMP kinase) at pH 8.0

Sample:	DTMP kinase dimer, 57 kDa Brugia malayi protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Oct 7

Crystal Structure of the Brugia malayi Thymidylate Kinase-dTMP Complex and Small Angle X-ray Scattering Experiments Identifies Changes in the Dimeric Association Compared to the Human Homolog Crystallography Reports 68(7):1150-1158 (2024)
Vishwakarma J, Sharma V, Kumar S, Ramachandran R

R_g^Guinier	3.0	nm
D_max	8.4	nm
Volume^Porod	81	nm³

SASDKL8 – S10 carboxypeptidase from Deinococccus radiodurans (S10drt1)

Carboxypeptidase-related protein experimental SAS data

Sample:	Carboxypeptidase-related protein 16-mer, 903 kDa Deinococcus radiodurans (strain … protein
Buffer:	20 mM Tris-Cl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Dec 25

Novel oligomeric assembly of S10-carboxypeptidase from Deinococcus radiodurans
Dr. Rahul Singh

R_g^Guinier	7.4	nm
D_max	16.8	nm
Volume^Porod	1180	nm³

SASDT29 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data

CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27

Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
Bennett AL, Edwards R, Kosheleva I, Saunders C, Bililign Y, Williams A, Bubphamala P, Manosouri K, Anasti K, Saunders KO, Alam SM, Haynes BF, Acharya P, Henderson R

R_g^Guinier	4.6	nm
D_max	15.5	nm
Volume^Porod	705	nm³

SASDT39 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 35 °C

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27

R_g^Guinier	4.6	nm
D_max	15.4	nm
Volume^Porod	704	nm³

SASDT49 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 44 °C

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27

R_g^Guinier	4.6	nm
D_max	15.6	nm
Volume^Porod	702	nm³

SASDT59 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 50 °C

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29

R_g^Guinier	4.6	nm
D_max	15.6	nm
Volume^Porod	699	nm³

SASDT69 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1.5 µs post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30

R_g^Guinier	4.6	nm
D_max	15.5	nm
Volume^Porod	706	nm³

SASDT79 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 3 µs post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30

R_g^Guinier	4.6	nm
D_max	15.5	nm
Volume^Porod	706	nm³

SASDT89 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 5 µs post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30

R_g^Guinier	4.6	nm
D_max	15.5	nm
Volume^Porod	706	nm³

SASDT99 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 µs post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28

R_g^Guinier	4.3	nm
D_max	15.3	nm
Volume^Porod	729	nm³

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