Small Angle Scattering Biological Data Bank SASBDB

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SASDS64 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram

Sample:	Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer:	20 mM Tris, 3 M GdnHCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Sep 15

Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
Koren G, Meir S, Holschuh L, Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R

R_g^Guinier

1.1

SASDRT5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ADP

Nitrogen fixation regulatory protein (Q409L) experimental SAS data

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J

R_g^Guinier	4.9	nm
D_max	17.9	nm
Volume^Porod	233	nm³

SASDRU5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ATP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	4.9	nm
D_max	18.0	nm
Volume^Porod	225	nm³

SASDRV5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ADP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	4.9	nm
D_max	18.0	nm
Volume^Porod	243	nm³

SASDRW5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ATP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	5.1	nm
D_max	17.8	nm
Volume^Porod	224	nm³

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain

Transient receptor potential cation channel subfamily V member 4 experimental SAS data

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U

R_g^Guinier	2.3	nm
D_max	11.5	nm
Volume^Porod	41	nm³

SASDQF8 – G. gallus TRPV4 N-terminal Domain

G. gallus TRPV4 N-terminal Domain Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.7	nm
D_max	19.0	nm
Volume^Porod	59	nm³

SASDQG8 – G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111

G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 42 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	4.0	nm
D_max	19.5	nm
Volume^Porod	78	nm³

SASDQH8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 37 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	64	nm³

SASDQJ8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 33 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.5	nm
D_max	11.9	nm
Volume^Porod	41	nm³

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