Browse by ORGANISM: other species

SASDJG7 – SAM2 Riboswitch 1 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

DUF507 family protein experimental SAS data
CUSTOM IN-HOUSE model
Sample: DUF507 family protein monomer, 22 kDa Aquifex aeolicus (strain … protein
Buffer: 20 mM Hepes pH 7.2, 400 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 7
Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold. Sci Rep 13(1):13496 (2023)
McKay CE, Cheng J, Tanner JJ
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 39 nm3

SASDJB6 – Lactococcus phage Phi28 apo-gp11 encapsidation protein

gp11 encapsidation protein experimental SAS data
OTHER model
Sample: Gp11 encapsidation protein decamer, 446 kDa Lactococcus phage Phi28 protein
Buffer: 100 mM NaCl, 25 mM HEPES, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2015 May 6
Lactococcus Phage ASCC Phi28 gp11
Mark White
RgGuinier 5.3 nm
Dmax 16.3 nm

SASDSA5 – dark-state short-linker LOV-activated diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 102 nm3

SASDSB5 – light-state short-linker LOV-activated diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 15.7 nm
VolumePorod 104 nm3

SASDSC5 – dark-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
GASBOR model
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 115 nm3

SASDSD5 – light-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
Sensor domain-containing diguanylate cyclase Kratky plot
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.5 nm
Dmax 13.8 nm
VolumePorod 123 nm3