Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDML3 – Mycobacterium phage TipsytheTRex immunity repressor bound to 24mer DNA

Immunity repressor24mer dsDNA experimental SAS data

Sample:	Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 24mer dsDNA monomer, 15 kDa DNA
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10

A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR

R_g^Guinier	2.4	nm
D_max	9.0	nm
Volume^Porod	43	nm³

SASDMM3 – Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA

Immunity repressor13mer dsDNA experimental SAS data

Sample:	Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 13mer dsDNA monomer, 8 kDa DNA
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 10

R_g^Guinier	2.0	nm
D_max	8.5	nm
Volume^Porod	18	nm³

SASDLF4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
Orr C, Fisher H, Yu X, Chan C, Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I

R_g^Guinier	3.9	nm
D_max	14.2	nm
Volume^Porod	135	nm³

SASDLG4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.2	nm
D_max	15.1	nm
Volume^Porod	135	nm³

SASDLH4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C225S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	131	nm³

SASDLJ4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant

ChiLob 7/4 IgG2 F(ab)2 C228S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C228S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.1	nm
D_max	13.5	nm
Volume^Porod	135	nm³

SASDLK4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	13.3	nm
Volume^Porod	133	nm³

SASDLL4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	13.7	nm
Volume^Porod	130	nm³

SASDJX7 – Labrum-Interacting Proteins from Saliva 2 (34k-2) - SEC-SAXS

Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 experimental SAS data

Sample:	Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 monomer, 35 kDa Aedes albopictus protein
Buffer:	25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Sep 3

A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum. Curr Biol (2022)
Arnoldi I, Mancini G, Fumagalli M, Gastaldi D, D'Andrea L, Bandi C, Di Venere M, Iadarola P, Forneris F, Gabrieli P

R_g^Guinier	2.7	nm
D_max	8.4	nm
Volume^Porod	50	nm³

SASDLC9 – 2-aminomuconic 6-semialdehyde dehydrogenase double truncation mutant (Δ124-138; Δ477-491)

2-aminomuconic 6-semialdehyde dehydrogenase experimental SAS data

Sample:	2-aminomuconic 6-semialdehyde dehydrogenase dimer, 101 kDa Pseudomonas sp. protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1

The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding. Environ Microbiol 24(7):2994-3012 (2022)
Shi Q, Chen Y, Li X, Dong H, Chen C, Zhong Z, Yang C, Liu G, Su D

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	117	nm³

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