Browse by ORGANISM: other species

SASDMC4 – Plasmodium falciparum tyrosyl-tRNA synthetase

Tyrosine--tRNA ligase experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S, Kosasih C, Newman W, Niles J, Lee M, Baum J, Ottilie S, Winzeler E, Creek D, Williamson N, Parker M, Brand S, Langston S, Dick L, Griffin M, Gould A, Tilley L
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 126 nm3

SASDMD4 – Plasmodium falciparum tyrosyl-tRNA synthetase, S234C mutant

Tyrosine--tRNA ligase (S234C) experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase (S234C) dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S, Kosasih C, Newman W, Niles J, Lee M, Baum J, Ottilie S, Winzeler E, Creek D, Williamson N, Parker M, Brand S, Langston S, Dick L, Griffin M, Gould A, Tilley L
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 124 nm3

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

Tegument protein UL21 (C-terminal domain) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 49 nm3

SASDPF2 – Structure of an extracellular superoxide dismutase from Onchocerca volvulus

Extracellular superoxide dismutase [Cu-Zn] experimental SAS data
GASBOR model
Sample: Extracellular superoxide dismutase [Cu-Zn] dimer, 34 kDa Onchocerca volvulus protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development Acta Crystallographica Section F Structural Biology Communications 78(6) (2022)
Moustafa A, Perbandt M, Liebau E, Betzel C, Falke S
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 55 nm3

SASDPB9 – SAXS analysis of pilus adhesin PitA from pilus islet-2 of Streptococcus oralis

von Willebrand factor type A domain protein experimental SAS data
MODELLER model
Sample: Von Willebrand factor type A domain protein monomer, 89 kDa Streptococcus oralis ATCC … protein
Buffer: 20 mM HEPES,150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 12
New structural insights into the PI-2 pilus from Streptococcus oralis, an early dental plaque colonizer. FEBS J (2022)
Yadav RK, Krishnan V
RgGuinier 6.4 nm
Dmax 29.2 nm
VolumePorod 217 nm3

SASDLP8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Extended

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Extended Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 72 nm3

SASDLQ8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Compact

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Compact Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 80 nm3

SASDL58 – The Fe–S cluster assembly 1 homolog of pigeon before applying the external magnetic field (0 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.7 nm
Dmax 15.8 nm

SASDL68 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 5 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.0 nm
Dmax 16.2 nm

SASDL78 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 10 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.9 nm
Dmax 16.5 nm