|
|
|
Sample: |
Clostridium butyricum argonaute protein monomer, 86 kDa protein
|
Buffer: |
20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
|
Argonaute protein SAXS investigation
lirong zheng
|
RgGuinier |
3.2 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
188 |
nm3 |
|
|
|
|
|
Sample: |
Clostridium butyricum argonaute protein monomer, 86 kDa protein
|
Buffer: |
20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
|
Argonaute protein SAXS investigation
lirong zheng
|
RgGuinier |
3.2 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
185 |
nm3 |
|
|
|
|
|
Sample: |
Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
|
Buffer: |
20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
|
Argonaute protein SAXS investigation
lirong zheng
|
RgGuinier |
3.1 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
122 |
nm3 |
|
|
|
|
|
Sample: |
Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
|
Buffer: |
20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
|
Argonaute protein SAXS investigation
lirong zheng
|
RgGuinier |
3.1 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
120 |
nm3 |
|
|
|
|
|
Sample: |
Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
|
Buffer: |
20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
|
Argonaute protein SAXS investigation
lirong zheng
|
RgGuinier |
3.3 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
175 |
nm3 |
|
|
|
|
|
Sample: |
RNA recognition motif (RRM)-containing protein 4 monomer, 111 kDa Ustilago maydis protein
|
Buffer: |
20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2021 Apr 30
|
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes
PLOS Genetics 18(6):e1010269 (2022)
Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
|
RgGuinier |
8.8 |
nm |
Dmax |
30.7 |
nm |
VolumePorod |
587 |
nm3 |
|
|
|
|
|
Sample: |
RNA recognition motif (RRM)-containing protein 4 NT4 monomer, 40 kDa Ustilago maydis protein
|
Buffer: |
20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2021 Apr 30
|
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes
PLOS Genetics 18(6):e1010269 (2022)
Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
|
RgGuinier |
5.6 |
nm |
Dmax |
18.5 |
nm |
VolumePorod |
123 |
nm3 |
|
|
|
|
|
Sample: |
Mycocerosic acid synthase monomer, 224 kDa Mycobacterium bovis (strain … protein
|
Buffer: |
50 mM Tris-HCl, 50 mM NaCl, 10% glycerol, pH: 8 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2013 Oct 1
|
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.
BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
|
RgGuinier |
5.7 |
nm |
Dmax |
21.0 |
nm |
VolumePorod |
420 |
nm3 |
|
|
|
|
|
Sample: |
Phenolpthiocerol synthesis type-I polyketide synthase ppsA monomer, 199 kDa Mycobacterium bovis (strain … protein
|
Buffer: |
50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH: 8 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2012 Jun 14
|
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.
BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
|
RgGuinier |
6.7 |
nm |
Dmax |
20.2 |
nm |
VolumePorod |
680 |
nm3 |
|
|
|
|
|
Sample: |
Botulinum Toxin Serotype E, endonegative monomer, 144 kDa Clostridium botulinum protein
|
Buffer: |
10 mM sodium acetate (20 mM ionic strength), pH: 4 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Oct 6
|
Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E.
J Struct Biol :107876 (2022)
Lalaurie CJ, Splevins A, Barata TS, Bunting KA, Higazi DR, Zloh M, Spiteri VA, Perkins SJ, Dalby PA
|
RgGuinier |
4.3 |
nm |
Dmax |
15.2 |
nm |
VolumePorod |
240 |
nm3 |
|
|