Small Angle Scattering Biological Data Bank SASBDB

SASDJC7 – SAM2 Riboswitch 2 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data

S-adenosylmethionine riboswitch II Kratky plot

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.2

SASDJD7 – SAM2 Riboswitch 3 mM MgCl2

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.1

SASDJE7 – SAM2 Riboswitch 3.75 mM MgCl2

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.1

SASDJF7 – SAM2 Riboswitch 0.25 mM MgCl2 Saturating SAM

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.25 mM MgCl2 SAM, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.7

SASDJG7 – SAM2 Riboswitch 1 mM MgCl2 Saturating SAM

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1 mM MgCl2 SAM, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.0

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.0

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.0

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.0

SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

DUF507 family protein experimental SAS data

Sample:	DUF507 family protein monomer, 22 kDa Aquifex aeolicus (strain … protein
Buffer:	20 mM Hepes pH 7.2, 400 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 7

Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold. Sci Rep 13(1):13496 (2023)
McKay CE, Cheng J, Tanner JJ

R_g^Guinier	2.3	nm
D_max	9.0	nm
Volume^Porod	39	nm³

SASDSF2 – K48-linked diubiquitin

Sample:	Polyubiquitin-C monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Feb 1

Stretching the chains: the destabilizing impact of Cu(2+) and Zn(2+) ions on K48-linked diubiquitin. Dalton Trans (2023)
Mangini V, Grasso G, Belviso BD, Sciacca MFM, Lanza V, Caliandro R, Milardi D

R_g^Guinier	1.9	nm
D_max	6.5	nm
Volume^Porod	22	nm³

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