Small Angle Scattering Biological Data Bank SASBDB

SASDQS4 – Escherichia coli 6S:pRNA complex

6S RNA (SsrS gene)product RNA from E. coli 6S experimental SAS data

Escherichia coli 6S:pRNA complex Rg histogram

Sample:	6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA product RNA from E. coli 6S monomer, 7 kDa Escherichia coli RNA
Buffer:	20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Apr 4

Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Frontiers in Molecular Biosciences 10 (2023)
Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	260	nm³

SASDRT5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ADP

Nitrogen fixation regulatory protein (Q409L) experimental SAS data

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J

R_g^Guinier	4.9	nm
D_max	17.9	nm
Volume^Porod	233	nm³

SASDRU5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ATP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	4.9	nm
D_max	18.0	nm
Volume^Porod	225	nm³

SASDRV5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ADP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	4.9	nm
D_max	18.0	nm
Volume^Porod	243	nm³

SASDRW5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ATP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	5.1	nm
D_max	17.8	nm
Volume^Porod	224	nm³

SASDM43 – RRM1-ZnF1 tandem domains of RNA-binding protein 5 (C191G mutant)

RNA-binding protein 5 (I107T, C191G) experimental SAS data

Sample:	RNA-binding protein 5 (I107T, C191G) monomer, 14 kDa Homo sapiens protein
Buffer:	20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Sep 26

Structural basis for specific RNA recognition by the alternative splicing factor RBM5. Nat Commun 14(1):4233 (2023)
Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	28	nm³

SASDM53 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant)

RNA Binding Motif protein 5 (I107T, C191G) experimental SAS data

RNA Binding Motif protein 5 (I107T, C191G) Kratky plot

Sample:	RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Feb 9

R_g^Guinier	2.3	nm
D_max	7.8	nm
Volume^Porod	36	nm³

SASDM63 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant) in complex with GGCU_12 RNA

RNA Binding Motif protein 5 (I107T, C191G)Caspase-2 derived RNA GGCU_12 experimental SAS data

Sample:	RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein Caspase-2 derived RNA GGCU_12 monomer, 4 kDa RNA
Buffer:	20 mM MES, 100 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Mar 1

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	42	nm³

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain

Transient receptor potential cation channel subfamily V member 4 experimental SAS data

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U

R_g^Guinier	2.3	nm
D_max	11.5	nm
Volume^Porod	41	nm³

SASDQF8 – G. gallus TRPV4 N-terminal Domain

G. gallus TRPV4 N-terminal Domain Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.7	nm
D_max	19.0	nm
Volume^Porod	59	nm³

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