SASDJ36 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDFY9 – Sperm-associated antigen 1 (SPAG1)

Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 7.3 nm
Dmax 34.0 nm
VolumePorod 374 nm3

SASDFZ9 – RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)

RuvB-like 1RuvB-like 2Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 6.8 nm
Dmax 34.8 nm
VolumePorod 1800 nm3

SASDG22 – RuvB-like 1 and 2 dodecamer (His-RuvBL1/RuvBL2)

RuvB-like 1RuvB-like 2 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 3
R2SP
Raphael Dos Santos Morais
RgGuinier 6.3 nm
Dmax 23.4 nm
VolumePorod 1550 nm3

SASDG32 – Sperm-associated antigen 1, PIH1 domain-containing protein 2 heterodimer complex (SPAG1/PIH1D2)

Sperm-associated antigen 1PIH1 domain-containing protein 2 experimental SAS data
DAMMIF model
Sample: Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
PIH1 domain-containing protein 2 monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 30.9 nm
VolumePorod 664 nm3

SASDG42 – Truncated sperm-associated antigen 1 (622-926), PIH1 domain-containing protein 2 heterodimer complex (SPAG1(622-926)/PIH1D2)

PIH1 domain-containing protein 2Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: PIH1 domain-containing protein 2 monomer, 36 kDa Homo sapiens protein
Sperm-associated antigen 1 monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 5.0 nm
Dmax 19.3 nm
VolumePorod 123 nm3

SASDR89 – Aromatic-L-amino-acid decarboxylase (AADC) - apo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency Protein Science (2023)
Bisello G, Ribeiro R, Perduca M, Belviso B, Polverino de' Laureto P, Giorgetti A, Caliandro R, Bertoldi M
RgGuinier 3.4 nm
Dmax 15.8 nm
VolumePorod 181 nm3

SASDR99 – Aromatic-L-amino-acid decarboxylase (AADC) - pyridoxal 5'-phosphate bound holo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency Protein Science (2023)
Bisello G, Ribeiro R, Perduca M, Belviso B, Polverino de' Laureto P, Giorgetti A, Caliandro R, Bertoldi M
RgGuinier 3.2 nm
Dmax 14.9 nm
VolumePorod 180 nm3

SASDRA9 – Aromatic-L-amino-acid decarboxylase (AADC) - dopa methylester DME-bound holo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data
DAMMIN model
Sample: Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Feb 28
Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency Protein Science (2023)
Bisello G, Ribeiro R, Perduca M, Belviso B, Polverino de' Laureto P, Giorgetti A, Caliandro R, Bertoldi M
RgGuinier 3.0 nm
Dmax 14.5 nm
VolumePorod 154 nm3

SASDQR4 – Escherichia coli 6S RNA

6S RNA (SsrS gene) experimental SAS data
Escherichia coli 6S RNA Rg histogram
Sample: 6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Buffer: 20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 4
Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Frontiers in Molecular Biosciences 10 (2023)
Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V
RgGuinier 6.1 nm
Dmax 24.0 nm
VolumePorod 175 nm3

4692 hits found.