Small Angle Scattering Biological Data Bank SASBDB

SASDP69 – Acinetobacter baumannii carbonic anhydrase PaaY trimer

Bacterial transferase hexapeptide repeat protein experimental SAS data

Sample:	Bacterial transferase hexapeptide repeat protein trimer, 66 kDa Acinetobacter baumannii (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 29

Mechanistic and structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure (2023)
Jiao M, He W, Ouyang Z, Qin Q, Guo Y, Zhang J, Bai Y, Guo X, Yu Q, She J, Hwang PM, Zheng F, Wen Y

R_g^Guinier	2.6	nm
D_max	9.0	nm
Volume^Porod	102	nm³

SASDRN9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data

Sample:	Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 45 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2

Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
Ehrit J, Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N

R_g^Guinier	3.4	nm
D_max	8.6	nm

SASDRP9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data

Sample:	Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 45 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2

R_g^Guinier	3.5	nm
D_max	8.8	nm

SASDRQ9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

$Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data$

Sample:	Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 20-mer, 24 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

R_g^Guinier	3.0	nm
D_max	8.0	nm

SASDRR9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

$Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data$

Sample:	Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 22-mer, 26 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

R_g^Guinier	3.1	nm
D_max	8.3	nm

SASDRS9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

$Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data$

Sample:	Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 42 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29

R_g^Guinier	3.3	nm
D_max	8.3	nm

SASDRT9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

$Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data$

Sample:	Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 42 kDa
Buffer:	aqueous solution of 4% methanol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29

R_g^Guinier	3.5	nm
D_max	8.7	nm

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

Recombination protein 107 experimental SAS data

Sample:	Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C

R_g^Guinier	8.3	nm
D_max	32.2	nm

SASDSQ2 – Sordaria macrospora ASY2 coiled-coiled with N-terminal SUMO-tag

WGS project CABT00000000 data, contig 2.12 experimental SAS data

Sample:	WGS project CABT00000000 data, contig 2.12 tetramer, 155 kDa Sordaria macrospora (strain … protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	9.6	nm
D_max	38.0	nm

SASDSR2 – Zea mays PAIR1 coiled-coiled with N-terminal SUMO-tag

Sample:	Protein PAIR1 tetramer, 132 kDa Zea mays protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	8.0	nm
D_max	31.7	nm

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