SASDNB3 – Homodimerisation of the CP110 C-terminus coiled-coil domain

Centriolar coiled-coil protein of 110 kDa experimental SAS data
DAMMIF model
Sample: Centriolar coiled-coil protein of 110 kDa dimer, 20 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 23
Microtubule plus-end regulation by centriolar cap proteins (2021)
Ogunmolu F, Moradi S, Volkov V, van Hoorn C, Wu J, Andrea N, Hua S, Jiang K, Vakonakis I, Potocnjak M, Herzog F, Gigant B, Gudimchuk N, Stecker K, Dogterom M, Steinmetz M, Akhmanova A
RgGuinier 3.5 nm
Dmax 12.5 nm
VolumePorod 33 nm3

SASDMK7 – HomA outer membrane protein

HomA outer membrane protein experimental SAS data
DAMMIN model
Sample: HomA outer membrane protein dimer, 146 kDa Helicobacter pylori protein
Buffer: 20 mM Tris-Cl, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2021 Feb 2
Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori. Sci Rep 11(1):24471 (2021)
Tamrakar A, Singh R, Kumar A, Makde RD, Ashish, Kodgire P
RgGuinier 8.3 nm
Dmax 28.2 nm
VolumePorod 426 nm3

SASDML7 – HomB outer membrane protein

HomB outer membrane protein experimental SAS data
DAMMIN model
Sample: HomB outer membrane protein dimer, 148 kDa Helicobacter pylori protein
Buffer: 20 mM Tris-Cl, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2021 Feb 2
Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori. Sci Rep 11(1):24471 (2021)
Tamrakar A, Singh R, Kumar A, Makde RD, Ashish, Kodgire P
RgGuinier 8.2 nm
Dmax 27.1 nm
VolumePorod 500 nm3

SASDD97 – Synthetic FRET biosensor Twitch 2B Ca-bound

Synthetic FRET biosensor Twitch 2B experimental SAS data
Synthetic FRET biosensor Twitch 2B Kratky plot
Sample: Synthetic FRET biosensor Twitch 2B monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 5 mM CaCl2, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 17
Synthetic FRET biosensor Twitch 2B
Pablo Trigo Mourino
RgGuinier 2.7 nm

SASDDA7 – Synthetic FRET biosensor Twitch 2B Ca-free

Synthetic FRET biosensor Twitch 2B experimental SAS data
Synthetic FRET biosensor Twitch 2B Kratky plot
Sample: Synthetic FRET biosensor Twitch 2B monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 17
Synthetic FRET biosensor Twitch 2B
Pablo Trigo Mourino
RgGuinier 4.1 nm

SASDDB7 – Synthetic FRET biosensor Twitch 2B N532F Ca-bound

Synthetic FRET biosensor Twitch2B N532F experimental SAS data
Synthetic FRET biosensor Twitch2B N532F Kratky plot
Sample: Synthetic FRET biosensor Twitch2B N532F monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 5 mM CaCl2, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 30
Synthetic FRET biosensor Twitch 2B
Pablo Trigo Mourino
RgGuinier 2.8 nm

SASDDC7 – Synthetic FRET biosensor Twitch 2B N532F Ca-free

Synthetic FRET biosensor Twitch2B N532F experimental SAS data
Synthetic FRET biosensor Twitch2B N532F Kratky plot
Sample: Synthetic FRET biosensor Twitch2B N532F monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 30
Synthetic FRET biosensor Twitch 2B
Pablo Trigo Mourino
RgGuinier 3.9 nm

SASDMR6 – 5-10-5 gapmer phosphorothioate antisense oligonucleotide

5-10-5 gapmer phosphorothioate antisense oligonucleotide experimental SAS data
DAMFILT model
Sample: 5-10-5 gapmer phosphorothioate antisense oligonucleotide dimer, 14 kDa
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 1.8 nm
Dmax 8.5 nm
VolumePorod 15 nm3

SASDMS6 – Non-POU domain-containing octamer-binding protein (NONO homodimer)

Non-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 96 nm3

SASDMT6 – Non-POU domain-containing octamer-binding protein (NONO homodimer) bound to an antisense oligonucleotide

Non-POU domain-containing octamer-binding protein5-10-5 gapmer phosphorothioate antisense oligonucleotide tetramer experimental SAS data
SASREF model
Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
5-10-5 gapmer phosphorothioate antisense oligonucleotide tetramer tetramer, 28 kDa
Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1. Nucleic Acids Res (2021)
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
RgGuinier 3.9 nm
Dmax 18.4 nm
VolumePorod 153 nm3

4122 hits found.