Small Angle Scattering Biological Data Bank SASBDB

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236

Outer membrane protein MIP experimental SAS data

SREFLEX model

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U

R_g^Guinier	3.1	nm
D_max	9.4	nm
Volume^Porod	63	nm³

SASDPB5 – SAXS curve of RNA Xist A-repeats (Liu buffer)

Xist A-repeat lncRNA experimental SAS data

Xist A-repeat lncRNA Kratky plot

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	25 mM K-HEPES, 0.1 mM Na-EDTA, 150 mM KCl, 15 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	12.1	nm
D_max	45.0	nm

SASDPC5 – SAXS curve of RNA Xist A-repeats (Smola buffer)

Xist A-repeat lncRNA experimental SAS data

Xist A-repeat lncRNA Kratky plot

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	100 mM HEPES, 100 mM NaCl, 10 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	9.8	nm
D_max	40.0	nm
Volume^Porod	657	nm³

SASDPD5 – SAXS curve of RNA Xist A-repeats (Maenner buffer)

Xist A-repeat lncRNA experimental SAS data

Xist A-repeat lncRNA Kratky plot

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	20 mM HEPES-KOH, 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 20% glycerol, 3.25 mM MgCl2, pH: 7.9
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	8.9	nm
D_max	35.0	nm
Volume^Porod	414	nm³

SASDPE5 – SAXS curve of RNA Xist A-repeats (Duszczyk buffer)

Xist A-repeat lncRNA experimental SAS data

Xist A-repeat lncRNA Kratky plot

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	10 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 0.02 mM EDTA, 0.02% azide, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	7.6	nm
D_max	30.0	nm
Volume^Porod	370	nm³

SASDPF5 – SAXS curve of RNA Xist A-repeats (14mer)

Xist A-repeat lncRNA 14 mer experimental SAS data

Xist A-repeat lncRNA 14 mer Kratky plot

Sample:	Xist A-repeat lncRNA 14 mer monomer, 5 kDa RNA
Buffer:	50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	13	nm³

SASDPG5 – SAXS curve of RNA Xist A-repeats (26mer)

Xist A-repeat lncRNA 24 mer experimental SAS data

Xist A-repeat lncRNA 24 mer Kratky plot

Sample:	Xist A-repeat lncRNA 24 mer dimer, 16 kDa RNA
Buffer:	50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	3.1	nm
D_max	14.5	nm
Volume^Porod	28	nm³

SASDPH5 – SAXS curve of RNA Xist A-repeats (1 repeat)

Xist A-repeat lncRNA 1 repeat experimental SAS data

Xist A-repeat lncRNA 1 repeat Kratky plot

Sample:	Xist A-repeat lncRNA 1 repeat dimer, 41 kDa RNA
Buffer:	50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	3.9	nm
D_max	16.0	nm
Volume^Porod	42	nm³

SASDPJ5 – SAXS curve of RNA Xist A-repeats (2 repeats)

Xist A-repeat lncRNA 2 repeats experimental SAS data

Xist A-repeat lncRNA 2 repeats Kratky plot

Sample:	Xist A-repeat lncRNA 2 repeats monomer, 34 kDa RNA
Buffer:	50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	5.0	nm
D_max	23.0	nm
Volume^Porod	87	nm³

SASDPK5 – SAXS curve of RNA Xist A-repeats (3 repeats)

Xist A-repeat lncRNA 3 repeats experimental SAS data

Xist A-repeat lncRNA 3 repeats Kratky plot

Sample:	Xist A-repeat lncRNA 3 repeats monomer, 48 kDa RNA
Buffer:	50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	6.3	nm
D_max	22.0	nm
Volume^Porod	97	nm³

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