|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.2 |
nm |
| Dmax |
11.1 |
nm |
| VolumePorod |
114 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.0 |
nm |
| Dmax |
10.5 |
nm |
| VolumePorod |
132 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 dimer, 215 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
5.7 |
nm |
| Dmax |
24.6 |
nm |
| VolumePorod |
389 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 dimer, 215 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
5.8 |
nm |
| Dmax |
24.6 |
nm |
| VolumePorod |
450 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 dimer, 215 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
5.3 |
nm |
| Dmax |
25.1 |
nm |
| VolumePorod |
298 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
4.1 |
nm |
| Dmax |
15.0 |
nm |
| VolumePorod |
273 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.8 |
nm |
| Dmax |
15.4 |
nm |
| VolumePorod |
226 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
|
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.9 |
nm |
| Dmax |
16.0 |
nm |
| VolumePorod |
230 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Draxin monomer, 45 kDa Homo sapiens protein
|
| Buffer: |
20 mM HEPES 150 mM NaCl, pH: 7.5
|
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2015 Aug 19
|
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC.
Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
|
| RgGuinier |
4.2 |
nm |
| Dmax |
15.0 |
nm |
| VolumePorod |
87 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
hypothetical protein CTHT_0072540 tetramer, 62 kDa Chaetomium thermophilum protein
|
| Buffer: |
20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
|
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jul 30
|
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.
Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
|
| RgGuinier |
4.1 |
nm |
| Dmax |
16.2 |
nm |
| VolumePorod |
145 |
nm3 |
|
|
