Small Angle Scattering Biological Data Bank SASBDB

SASDAW5 – XMRV RT + DNA/RNA hybrid

apo XMRV RTRNA_DNA hybrid substrate experimental SAS data

Sample:	apo XMRV RT monomer, 75 kDa Escherichia coli protein RNA_DNA hybrid substrate monomer, 15 kDa
Buffer:	10 mM HEPES 100 mM KCl 5% Glycerol, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 8

Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid. Nucleic Acids Res 41(6):3874-87 (2013)
Nowak E, Potrzebowski W, Konarev PV, Rausch JW, Bona MK, Svergun DI, Bujnicki JM, Le Grice SF, Nowotny M

R_g^Guinier	3.5	nm
D_max	11.5	nm
Volume^Porod	155	nm³

SASDL47 – Oligomeric composition of AXH Domain of Ataxin-1 (wild type and A567G, I580A mutants)

Sample:	Ataxin-1 monomer, 14 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 21

Self-assembly and conformational heterogeneity of the AXH domain of ataxin-1: an unusual example of a chameleon fold. Biophys J 104(6):1304-13 (2013)
de Chiara C, Rees M, Menon RP, Pauwels K, Lawrence C, Konarev PV, Svergun DI, Martin SR, Chen YW, Pastore A

R_g^Guinier

5.5

SASDAF4 – DmMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data

Peroxisomal multifunctional enzyme type 2 Kratky plot

Sample:	Peroxisomal multifunctional enzyme type 2 dimer, 128 kDa Drosophila melanogaster protein
Buffer:	20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 12

Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
Mehtälä ML, Haataja TJ, Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T

R_g^Guinier	3.6	nm
D_max	12.0	nm

SASDAG4 – HsMfe2

Sample:	Peroxisomal multifunctional enzyme type 2 dimer, 159 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 22

R_g^Guinier	4.6	nm
D_max	15.0	nm

SASDDJ2 – Calcium-bound polcalcin Phl p 7

Sample:	polcalcin Phl p 7 monomer, 9 kDa Phleum pratense protein
Buffer:	0.15M NaCl, 0.025M Hepes, pH 7.4, 100 uM Ca2+, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2011 Dec 8

Solution structures of polcalcin Phl p 7 in three ligation states: Apo-, hemi-Mg2+-bound, and fully Ca2+-bound. Proteins 81(2):300-15 (2013)
Henzl MT, Sirianni AG, Wycoff WG, Tan A, Tanner JJ

R_g^Guinier	1.3	nm
D_max	3.6	nm
Volume^Porod	14	nm³

SASDBR4 – Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1)

Activator of Hsp90 ATPase-1 experimental SAS data

Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1) Rg histogram

Sample:	Activator of Hsp90 ATPase-1 monomer, 38 kDa Leishmania braziliensis protein
Buffer:	25 mM Sodium phosphate, 50 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, pH: 7
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 1

Low resolution structural studies indicate that the activator of Hsp90 ATPase 1 (Aha1) of Leishmania braziliensis has an elongated shape which allows its interaction with both N- and M-domains of Hsp9... PLoS One 8(6):e66822 (2013)
Seraphim TV, Alves MM, Silva IM, Gomes FE, Silva KP, Murta SM, Barbosa LR, Borges JC

R_g^Guinier	3.6	nm
D_max	14.5	nm

SASDHY2 – Human APPL2 (DCC-interacting protein 13-beta)

Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 experimental SAS data

Sample:	Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 dimer, 87 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 7 Apr 20

Membrane curvature protein exhibits interdomain flexibility and binds a small GTPase. J Biol Chem 287(49):40996-1006 (2012)
King GJ, Stöckli J, Hu SH, Winnen B, Duprez WG, Meoli CC, Junutula JR, Jarrott RJ, James DE, Whitten AE, Martin JL

R_g^Guinier	5.1	nm
D_max	18.0	nm
Volume^Porod	140	nm³

SASDAT4 – uPAR wild-type

Urokinase plasminogen activator surface receptor experimental SAS data

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M

R_g^Guinier	2.8	nm
D_max	9.7	nm
Volume^Porod	69	nm³

SASDAU4 – uPAR H47C/N259C

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	57	nm³

SASDAV4 – uPAR wild-type AE105 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE105 experimental SAS data

Urokinase plasminogen activator surface receptor synthetic peptide AE105 Kratky plot

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein synthetic peptide AE105 monomer, 1 kDa protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

R_g^Guinier	2.5	nm
D_max	8.5	nm
Volume^Porod	61	nm³

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