Standard proteins

Darja Ruskule.

SASDA62 – bAmylase in PBS

Beta-amylase
MWI(0) 162 kDa
MWexpected 224 kDa
VPorod 214 nm3
log I(s) 1.64×102 1.64×101 1.64×100 1.64×10-1
Beta-amylase small angle scattering data  s, nm-1
ln I(s)
Beta-amylase Guinier plot ln 1.64×102 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Beta-amylase Kratky plot 1.104 0 √3 sRg
p(r)
Beta-amylase pair distance distribution function Rg: 4.1 nm 0 Dmax: 12.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Beta-amylase DAMMIF model
log I(s)
 s, nm-1
Beta-amylase PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of bAmylase in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 12.5 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Beta-amylase
Mol. type   Protein
Organism   Ipomoea batatas
Olig. state   Tetramer
Mon. MW   56.1 kDa
 
UniProt   P10537
Sequence   FASTA
 
PDB ID   1FA2