| MWexperimental | 190 | kDa |
| MWexpected | 189 | kDa |
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log I(s)
1.77×100
1.77×10-1
1.77×10-2
1.77×10-3
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s, nm-1
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Structural determinants and mechanism of mammalian CRM1 allostery.
Dölker N, Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A, Structure 21(8):1350-60 (2013) Europe PMCSASDAL4 – CRM1 RanGTP Snu1
Exportin-1GTP-binding nuclear protein Ran
Snurportin-1
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Fits and models
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Synchrotron SAXS
data from solutions of
CRM1 RanGTP Snu1
in
50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1 and 7 mg/ml were measured
at 10°C.
One
120 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Wavelength = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN
Tags:
X33
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