Structural determinants and mechanism of mammalian CRM1 allostery.

Dölker N, Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A
Structure 21(8):1350-60 (2013 Aug 6)

PMID: 23850451 doi: 10.1016/j.str.2013.05.015

Published in SASBDB: 2015 Jul 7

SASDAJ4 – CRM1 Sample: Exportin-1 monomer, 123 kDa Mus musculus protein Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3 RgGuinier 3.8 nm Dmax 11.0 nm VolumePorod 190 nm3

SASDAK4 – CRM1 RanGTP Sample: Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3 RgGuinier 3.6 nm Dmax 10.0 nm

SASDAL4 – CRM1 RanGTP Snu1 Sample: Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein Snurportin-1 monomer, 41 kDa Homo sapiens protein Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3 RgGuinier 4.1 nm Dmax 14.0 nm

SASDAM4 – CRM1 Snu1 Sample: Exportin-1 monomer, 123 kDa Mus musculus protein Snurportin-1 monomer, 41 kDa Homo sapiens protein Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 10 RgGuinier 4.3 nm Dmax 15.0 nm