Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy.

Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M, J Am Chem Soc 130(20):6395-403 (2008) Europe PMC

SASDAN5 – Cross-linked complex CytC_Adr

Cytochrome C
Adrenodoxin
MWI(0) 23 kDa
MWexpected 22 kDa
VPorod 42 nm3
log I(s) 5.20×100 5.20×10-1 5.20×10-2 5.20×10-3
Cytochrome C Adrenodoxin small angle scattering data  s, nm-1
ln I(s)
Cytochrome C Adrenodoxin Guinier plot ln 5.20×100 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Cytochrome C Adrenodoxin Kratky plot 1.104 0 3 sRg
p(r)
Cytochrome C Adrenodoxin pair distance distribution function Rg: 2.2 nm 0 Dmax: 8.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Cytochrome C Adrenodoxin DAMMIN model

log I(s)
 s, nm-1
Cytochrome C Adrenodoxin SASREF model

Synchrotron SAXS data from solutions of Cross-linked complex CytC_Adr in 20 mM HEPES 2 mM DTT, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.4 and 24 mg/ml were measured at 15°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Cytochrome C (Cyt_C)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   11 kDa
Sequence   FASTA
 
Adrenodoxin (Adrenodoxin)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   11 kDa
Sequence   FASTA