Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy.

Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M
J Am Chem Soc 130(20):6395-403 (2008 May 21)
PMID: 18439013
doi: 10.1021/ja7101357
Published in SASBDB:

SASDAN5 – Cross-linked complex CytC_Adr

Cytochrome CAdrenodoxin experimental SAS data
DAMMIN model
Sample: Cytochrome C monomer, 11 kDa Escherichia coli protein
Adrenodoxin monomer, 11 kDa Escherichia coli protein
Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2006 Jun 15
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 42 nm3

SASDAP5 – Native complex CytC_Adr

Cytochrome C dimerAdrenodoxin dimer experimental SAS data
DAMMIN model
Sample: Cytochrome C dimer dimer, 22 kDa Escherichia coli protein
Adrenodoxin dimer dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2006 Jun 15
RgGuinier 2.9 nm
Dmax 9.5 nm
VolumePorod 64 nm3