Standard proteins

Darja Ruskule.

SASDAQ2 – Ubiquitin in sodium acetate

Ubiquitin
MWI(0) 8 kDa
MWexpected 9 kDa
VPorod 12 nm3
log I(s) 7.09×100 7.09×10-1 7.09×10-2 7.09×10-3
Ubiquitin small angle scattering data  s, nm-1
ln I(s)
Ubiquitin Guinier plot ln 7.09×100 Rg: 1.3 nm 0 (1.3 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitin Kratky plot 1.104 0 3 sRg
p(r)
Ubiquitin pair distance distribution function Rg: 1.3 nm 0 Dmax: 4.9 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ubiquitin PDB (PROTEIN DATA BANK) model

log I(s)
 s, nm-1
Ubiquitin DAMMIF model

Synchrotron SAXS data from solutions of Ubiquitin in sodium acetate in 40 mM Sodium acetate 150 mM NaCl, pH 5.5 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.20 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Concentration min = UNKNOWN

Tags: benchmark X33
Ubiquitin (UBQ)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   8.6 kDa
 
UniProt   P63048 (1-76)
Sequence   FASTA
 
PDB ID   1UBQ