Crystal Structure of an Engineered LRRTM2 Synaptic Adhesion Molecule and a Model for Neurexin Binding.

Paatero A, Rosti K, Shkumatov AV, Sele C, Brunello C, Kysenius K, Singha P, Jokinen V, Huttunen H, Kajander T, Biochemistry 55(6):914-26 (2016) Europe PMC

SASDBG3 – Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct)

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct)

MW^experimental	50	kDa
MW^expected	40	kDa

log I(s) 5.92×10¹ 5.92×10⁰ 5.92×10^-1 5.92×10^-2

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) Guinier plot

ln 5.92×10¹ R_g: 3.3 nm 0 (3.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.3 nm 0 D_max: 13.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0
0.831

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) DAMMIN model

X-ray synchrotron radiation scattering data from solutions of Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) in 20 mM Tris 150 mM NaCl, 3% glycerol were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 1.10-5.50 mg/ml were measured. 10 successive 1 second frames were collected at a sample temperature of 20 degrees centigrade. SAXS data from the samples and corresponding matched solvent blank were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a merged concentration scattering curve.

Cell temperature = UNKNOWN

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) (cLRRTM2 30-380)
Mol. type		Protein
Organism		Mus musculus
Olig. state		Monomer
Mon. MW		40.0 kDa

UniProt		Q8BGA3 (30-380)
Sequence		FASTA