Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.

Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ, FEBS J 2017 Jul 15; PubMed

SASDCP3 – Proline utilization A from Bdellovibrio bacteriovorus

Bifunctional protein PutA
MWexperimental 225 kDa
MWexpected 219 kDa
VPorod 287 nm3
log I(s) 1.13×103 1.13×102 1.13×101 1.13×100
Bifunctional protein PutA small angle scattering data  s, nm-1
ln I(s)
Bifunctional protein PutA Guinier plot ln 1.13×103 Rg: 4.5 nm 0 (4.5 nm)-2 s2
Bifunctional protein PutA Kratky plot 1.104 0 3 sRg
Bifunctional protein PutA pair distance distribution function Rg: 4.6 nm 0 Dmax: 14 nm

Experimental data validation

Fits and models

log I(s)
 s, nm-1
Bifunctional protein PutA DAMMIF model
Bifunctional protein PutA DAMFILT model
Synchrotron SAXS data from solutions of Proline utilization A from Bdellovibrio bacteriovorus in 50 mM Tris, 125 mM NaCl, 1 mM EDTA and 1 mM tris(3-hydroxypropyl)phosphine (THP) at pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector. The data were normalized to the intensity of the transmitted beam and radially averaged. The radially averaged scattering of the solvent-blank was subtracted.

Bifunctional protein PutA
Mol. type   Protein
Organism   Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Olig. state   Dimer
Mon. MW   109.5 kDa
UniProt   Q6MNK1
Sequence   FASTA