Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.

Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
FEBS J 284(18):3029-3049 (2017 Sep)

PMID: 28710792 doi: 10.1111/febs.14165

Submitted to SASBDB: 2017 May 19 Published in SASBDB: 2017 Jul 17

SASDCP3 – Proline utilization A from Bdellovibrio bacteriovorus Sample: Bifunctional protein PutA dimer, 219 kDa Bdellovibrio bacteriovorus protein Buffer: 50 mM Tris, 125 mM NaCl, 1 mM EDTA, and 1 mM tris(3-hydroxypropyl)phosphine (THP) at pH 7.5,, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8 RgGuinier 4.5 nm Dmax 14.0 nm VolumePorod 287 nm3

SASDCQ3 – Proline utilization A from Desulfovibrio vulgaris 1.5 mg/mL Sample: Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8 RgGuinier 4.4 nm Dmax 16.0 nm VolumePorod 293 nm3

SASDCR3 – Proline utilization A from Legionella pneumophila 3 mg/mL Sample: Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20 RgGuinier 4.6 nm Dmax 16.0 nm VolumePorod 291 nm3

SASDCS3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 2.3 mg/mL Sample: Proline dehydrogenase tetramer, 430 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16 RgGuinier 5.3 nm Dmax 14.1 nm VolumePorod 541 nm3

SASDCT3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 4.7 mg/mL Sample: Proline dehydrogenase tetramer, 430 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 12 RgGuinier 5.2 nm Dmax 14.6 nm VolumePorod 553 nm3

SASDCU3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 7.0 mg/mL Sample: Proline dehydrogenase tetramer, 430 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 12 RgGuinier 5.2 nm Dmax 13.7 nm VolumePorod 560 nm3

SASDCV3 – Proline utilization A from Legionella pneumophila 5 mg/mL Sample: Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20 RgGuinier 4.6 nm Dmax 15.3 nm VolumePorod 297 nm3

SASDCW3 – Proline utilization A from Legionella pneumophila 8 mg/mL Sample: Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20 RgGuinier 4.6 nm Dmax 15.5 nm VolumePorod 295 nm3

SASDCX3 – Proline utilization A from Desulfovibrio vulgaris 3.0 mg/mL Sample: Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8 RgGuinier 4.4 nm Dmax 16.0 nm VolumePorod 295 nm3

SASDCY3 – Proline utilization A from Desulfovibrio vulgaris 4.5 mg/mL Sample: Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8 RgGuinier 4.4 nm Dmax 16.0 nm VolumePorod 294 nm3

SASDCZ3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL Sample: Proline dehydrogenase dimer, 215 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16 RgGuinier 4.5 nm Dmax 14.5 nm VolumePorod 281 nm3

SASDC24 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 4.7 mg/mL Sample: Proline dehydrogenase dimer, 215 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16 RgGuinier 4.5 nm Dmax 13.9 nm VolumePorod 283 nm3

SASDC34 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL Sample: Proline dehydrogenase dimer, 215 kDa Bradyrhizobium diazoefficiens protein Buffer: 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16 RgGuinier 4.5 nm Dmax 14.6 nm VolumePorod 289 nm3