Synchrotron SAXS data from solutions of the multi-component estrogen receptor in 10 mM CHES, 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol, pH 9.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne, IL, USA) using a MAR 165 CCD detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.1 and 0.8 mg/ml were measured at 10°C from size-exclusion chromatography eluates. 20 successive 1.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Additional SEC parameters:
Column type: GE Healthcare Superdex 200 GL 10/300;
Flow rate: 0.5 ml/min;
Sample injection concentration: 3.5 mg/ml;
Injection volume: 500 µl.
|
Estrogen receptor
(hERa CDE)
|
Mol. type |
|
Protein |
Olig. state |
|
Dimer |
Mon. MW |
|
42.5 kDa |
|
UniProt |
|
P03372 (181-552) |
Sequence |
|
FASTA |
|
ERE1
(hERa_ERE1)
|
Mol. type |
|
DNA |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
5.9 kDa |
Sequence |
|
FASTA |
|
ERE2
(hERa_ERE2)
|
Mol. type |
|
DNA |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
5.9 kDa |
Sequence |
|
FASTA |
|
Estradiol
|
Mol. type |
|
Other |
Olig. state |
|
Dimer |
Mon. MW |
|
0.3 kDa |
Chemical formula |
|
hERa peptide1
|
Mol. type |
|
Protein |
Olig. state |
|
Monomer |
Mon. MW |
|
1.7 kDa |
Sequence |
|
FASTA |
|
hERa peptide2
|
Mol. type |
|
Protein |
Olig. state |
|
Monomer |
Mon. MW |
|
1.7 kDa |
Sequence |
|
FASTA |
|
|