cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity.

Liu H, Moura-Alves P, Pei G, Mollenkopf HJ, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler AB, Oberbeck-Mueller D, Hahnke K, Klemm M, Guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH, EMBO Rep (2019) Europe PMC

SASDEQ9 – Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP)

Cyclic GMP-AMP synthase
2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine
MWexperimental 98 kDa
MWexpected 124 kDa
VPorod 127 nm3
log I(s) 2.54×103 2.54×102 2.54×101 2.54×100
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine small angle scattering data  s, nm-1
ln I(s)
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine Guinier plot ln 2.54×103 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine Kratky plot 1.104 0 3 sRg
p(r)
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine pair distance distribution function Rg: 4.1 nm 0 Dmax: 14.1 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP) Rg histogram Rg, nm
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine EOM/RANCH model
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine EOM/RANCH model
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine EOM/RANCH model
Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine EOM/RANCH model

Synchrotron SAXS data from solutions of cyclic GMP-AMP synthase (cGAS) with 2'3'-cGAMP in 20 mM HEPES, pH 7.4 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column: XXXX ; Flow rate: 0.5 mL/min; Total acquisition time: 3600 x 1 s frames; Sample injection concentration: 9 mg/mL; Injection volume: 75 μL. The data obtained through the sample elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

Cyclic GMP-AMP synthase (cGAS)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   61.4 kDa
 
UniProt   Q8N884 (1-522)
Sequence   FASTA
 
2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine (2′3′-cGAMP)
Mol. type   Other
Olig. state   Dimer
Mon. MW   0.7 kDa
Chemical formula