Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations

Gijsbers A, Montagut D, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N, International Journal of Molecular Sciences 19(12):4012 (2018) DOI

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)

Ribosome maturation protein SDO1
MWexperimental 29 kDa
MWexpected 28 kDa
log I(s) 1.10×10-2 1.10×10-3 1.10×10-4 1.10×10-5
Ribosome maturation protein SDO1 small angle scattering data  s, nm-1
ln I(s)
Ribosome maturation protein SDO1 Guinier plot ln 1.10×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Ribosome maturation protein SDO1 Kratky plot 1.104 0 3 sRg
p(r)
Ribosome maturation protein SDO1 pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ribosome maturation protein SDO1 MULTIFOXS model
Ribosome maturation protein SDO1 MULTIFOXS model
Ribosome maturation protein SDO1 MULTIFOXS model

Synchrotron SAXS data from solutions of Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1) in 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 camera at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample elution peak (collected as 580 successive 3 second frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

SEC-SAXS was performed at 20°C using the following parameters: Column: Shodex KW-403 ; Flow rate: 0.408 mL/min; Total acquisition time: 1740s; Sample injection concentration: 10 mg/mL; Injection volume: 45μL.

Ribosome maturation protein SDO1 (SDO1)
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   28.3 kDa
 
UniProt   Q07953
Sequence   FASTA