Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations

Gijsbers A, Montagut D, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
International Journal of Molecular Sciences 19(12):4012 (2018 Dec 12)

doi: 10.3390/ijms19124012
Submitted to SASBDB: 2018 Dec 6
Published in SASBDB:

SASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3

Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome maturation protein SDO1 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 14
RgGuinier 2.1 nm
Dmax 6.2 nm
VolumePorod 25 nm3

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)

Ribosome maturation protein SDO1 experimental SAS data
MULTIFOXS model
Sample: Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae (strain ... protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
RgGuinier 2.7 nm
Dmax 8.5 nm

SASDEU8 – GTPase Elongation Factor like-1 protein (yeast EFL1)

Ribosome assembly protein 1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae (strain ... protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 258 nm3

SASDET8 – GTPase Elongation Factor like-1 bound Shwachman-Diamond Syndrome protein (EFL1*SDo1)

Ribosome assembly protein 1Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae (strain ... protein
Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae (strain ... protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 333 nm3