| MWexperimental | 136 | kDa |
| MWexpected | 117 | kDa |
| VPorod | 117 | nm3 |
|
log I(s)
8.15×100
8.15×10-1
8.15×10-2
8.15×10-3
|
s, nm-1
|
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair.
Khanam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R, Nucleic Acids Res (2020) Europe PMCSASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA
DNA ligase AProbable exodeoxyribonuclease III protein XthA
Nicked DNA
|
|
|
Fits and models
|
|
|
Synchrotron SAXS data from solutions of DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA in 50 mM Tris-HCl 200 mM NaCl 2mM β-mercaptoethanol (BME), pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.081 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||