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9 hits found for Ramachandran

SASDHU5 – Heat shock protein 70 (PfHSP70-1)

Heat shock protein 70Peptide experimental SAS data
DAMFILT model
Sample: Heat shock protein 70 monomer, 74 kDa Plasmodium falciparum protein
Peptide monomer, 1 kDa synthetic construct protein
Buffer: 50mM Tris, 5mM MgCl2, 500mM KCl, 5mM Bme, 5% glycerol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24
Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
...Ramachandran R, Kumar N
RgGuinier 5.3 nm
Dmax 16.6 nm
VolumePorod 450 nm3

SASDDD8 – class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III

Probable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: Probable exodeoxyribonuclease III protein XthA monomer, 32 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 12
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 2.4 nm
Dmax 7.3 nm
VolumePorod 56 nm3

SASDHR5 – Heat shock protein 40, subfamily A (PfHSP40)

HSP40, subfamily A experimental SAS data
DAMMIF model
Sample: HSP40, subfamily A dimer, 97 kDa Plasmodium falciparum protein
Buffer: 50 mM Tris, 5 mM MgCl2, 300 mM KCl, 1 mM β-mercaptoethanol, 5% glycerol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24
Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
...Ramachandran R, Kumar N
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 339 nm3

SASDDN9 – Mycobacterium tuberculosis DNA Ligase A

DNA ligase A experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 870 nm3

SASDDV9 – Mycobacterium tuberculosis DNA LigaseA with Nicked DNA

DNA ligase ANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Nicked DNA dimer, 16 kDa DNA
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 4.4 nm
Dmax 14.8 nm
VolumePorod 262 nm3

SASDEW3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA)

DNA ligase AProbable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 6.2 nm
Dmax 23.9 nm
VolumePorod 662 nm3

SASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA

DNA ligase AProbable exodeoxyribonuclease III protein XthANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
Nicked DNA monomer, 8 kDa DNA
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 4.5 nm
Dmax 17.1 nm
VolumePorod 117 nm3

SASDDQ8 – The complex formed between the class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III (XthA) bound to the BRCT domain from Mycobacterium tuberculosis DNA ligase

Probable exodeoxyribonuclease III protein XthAM. tb. LigA BRCT domain experimental SAS data
DAMFILT model
Sample: Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
M. tb. LigA BRCT domain monomer, 15 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 9
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 3.7 nm
Dmax 18.5 nm
VolumePorod 112 nm3

SASDD88 – The BRCT domain from Mycobacterium tuberculosis DNA ligase

M.tb. LigA BRCT domain (DNA ligase A) experimental SAS data
DAMMIN model
Sample: M.tb. LigA BRCT domain (DNA ligase A) monomer, 13 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Jun 2
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Ramachandran R
RgGuinier 1.6 nm
Dmax 3.7 nm
VolumePorod 23 nm3