M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair.

Khanam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
Nucleic Acids Res (2020 Mar 30)

PMID: 32232338 doi: 10.1093/nar/gkaa188

Submitted to SASBDB: 2018 Sep 6 Published in SASBDB: 2019 May 28

SASDDD8 – class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III Sample: Probable exodeoxyribonuclease III protein XthA monomer, 32 kDa Mycobacterium tuberculosis protein Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 May 12 RgGuinier 2.4 nm Dmax 7.3 nm VolumePorod 56 nm3

SASDDN9 – Mycobacterium tuberculosis DNA Ligase A Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 May 13 RgGuinier 5.2 nm Dmax 16.7 nm VolumePorod 870 nm3

SASDDV9 – Mycobacterium tuberculosis DNA LigaseA with Nicked DNA Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Nicked DNA dimer, 16 kDa DNA Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 May 13 RgGuinier 4.4 nm Dmax 14.8 nm VolumePorod 262 nm3

SASDEW3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 May 13 RgGuinier 6.2 nm Dmax 23.9 nm VolumePorod 662 nm3

SASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein Nicked DNA monomer, 8 kDa DNA Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 May 13 RgGuinier 4.5 nm Dmax 17.1 nm VolumePorod 117 nm3

SASDDQ8 – The complex formed between the class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III (XthA) bound to the BRCT domain from Mycobacterium tuberculosis DNA ligase Sample: Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein M. tb. LigA BRCT domain monomer, 15 kDa Mycobacterium tuberculosis protein Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 9 RgGuinier 3.7 nm Dmax 18.5 nm VolumePorod 112 nm3

SASDD88 – The BRCT domain from Mycobacterium tuberculosis DNA ligase Sample: M.tb. LigA BRCT domain (DNA ligase A) monomer, 13 kDa Mycobacterium tuberculosis protein Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Jun 2 RgGuinier 1.6 nm Dmax 3.7 nm VolumePorod 23 nm3