Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.

Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A, Proc Natl Acad Sci U S A (2019) Europe PMC

SASDF55 – Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody

Leukocidin G
Leukocidin H
LukGH neutralizing antibody
MWI(0) 90 kDa
MWexpected 121 kDa
VPorod 152 nm3
log I(s) 5.74×103 5.74×102 5.74×101 5.74×100
Leukocidin G Leukocidin H LukGH neutralizing antibody small angle scattering data  s, nm-1
ln I(s)
Leukocidin G Leukocidin H LukGH neutralizing antibody Guinier plot ln 5.75×103 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Leukocidin G Leukocidin H LukGH neutralizing antibody Kratky plot 1.104 0 3 sRg
p(r)
Leukocidin G Leukocidin H LukGH neutralizing antibody pair distance distribution function Rg: 4.9 nm 0 Dmax: 16 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Leukocidin G Leukocidin H LukGH neutralizing antibody PDB model

Synchrotron SAXS data from solutions of LukGH from Staphylococcus aureus bound to a neutralizing antibody in 20 mM HEPES, 300 mM NaCl, 1 mM MgCl2, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 4.6 mg/ml were measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Leukocidin G (LukG)
Mol. type   Protein
Organism   Staphylococcus aureus
Olig. state   Monomer
Mon. MW   35.6 kDa
 
UniProt   P21224 (30-338)
Sequence   FASTA
 
Leukocidin H (LukH)
Mol. type   Protein
Organism   Staphylococcus aureus
Olig. state   Monomer
Mon. MW   37.6 kDa
 
UniProt   Q5HEH9 (28-351)
Sequence   FASTA
 
LukGH neutralizing antibody (Fab)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   47.4 kDa
Sequence   FASTA
 
PDB code   5K59