Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.

Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A
Proc Natl Acad Sci U S A (2019 Dec 18)
PMID: 31852826
doi: 10.1073/pnas.1913690116
Submitted to SASBDB: 2019 Mar 28
Published in SASBDB:

SASDF45 – Leukocidin/Integrin alpha-M complex (LukGH-huCD11b-I) in the presence of a neutralizing antibody Fab fragment

Leukocidin GLeukocidin HLukGH neutralizing antibodyIntegrin alpha-M experimental SAS data
PYMOL model
Sample: Leukocidin G monomer, 36 kDa Staphylococcus aureus protein
Leukocidin H monomer, 38 kDa Staphylococcus aureus protein
LukGH neutralizing antibody monomer, 47 kDa Homo sapiens protein
Integrin alpha-M monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 300 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 17
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 178 nm3

SASDF55 – Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody

Leukocidin GLeukocidin HLukGH neutralizing antibody experimental SAS data
PDB model
Sample: Leukocidin G monomer, 36 kDa Staphylococcus aureus protein
Leukocidin H monomer, 38 kDa Staphylococcus aureus protein
LukGH neutralizing antibody monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 300 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 17
RgGuinier 4.7 nm
Dmax 16.0 nm
VolumePorod 152 nm3